ADVANTAGES OF PERIPLASMATIC EXPRESSION AND «CASSETTE» MUTAGENESIS OF RECOMBINANT PROTEINS TECHNOLOGY
https://doi.org/10.51523/2708-6011.2005-2-4-26
Abstract
Biotechnology methods allow getting homogenous biologically active preparations in virtually unlimited quantities. Recombinant protein purification technology is being constantly improved to increase expression level and minimization of number of protein purification stages. This paper introduces the new method of one-stage recombinant His-Tag fusion adrenodoxin cloning. It keeps all vector advantages for its periplasmatic expression. In this issue, optimized detergents system for effective lysis of bacteria cells has been described.
About the Authors
Y. G. Pohodnya
International Sakharov Environmental University
Belarus
Belarus
T. A. Skrahina
International Sakharov Environmental University
Belarus
Belarus
A. G. Lapko
International Sakharov Environmental University
Belarus
Belarus
References
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2. Wang W. and Malcolm B.A. Two-Stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Direction Mutagenesis // BioTechniques. - 1999. - Vol. 26. - № 4. - Р. 680-682.
Review
For citations:
Pohodnya Y.G., Skrahina T.A., Lapko A.G. ADVANTAGES OF PERIPLASMATIC EXPRESSION AND «CASSETTE» MUTAGENESIS OF RECOMBINANT PROTEINS TECHNOLOGY. Health and Ecology Issues. 2005;(4):131-136. (In Russ.) https://doi.org/10.51523/2708-6011.2005-2-4-26
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