Study of the potential of tolerogenic dendritic cells to immunophenotypic reversion affected by tumor necrosis factor
https://doi.org/10.51523/2708-6011.2025-22-2-08
Abstract
Objective. To assess reversion of tolerogenic dendritic cells (tolDC), obtained from human blood monocytes into immunogenic mature dendritic cells (mDC) under the influence of TNF-α.
Materials and methods. The immunophenotype of dendritic cells (DCs) obtained from blood monocytes of healthy donors (n=6) was assessed by flow cytometry: immature DC culture (immDC), mature (mDC) culture, tolDC culture, tolDC culture after exposure to the proinflammatory factor TNF-α (tol DCs/TNF-α). Statistical analysis was performed using nonparametric methods.
Results. There was 24.05(19.30-28.20) % of CD83+ cells in the population of mDCs, that was significantly higher compared with immDCs, tolDCs and tolDCs after stimulation with TNF-α (imm DCs – 4.10 (2.75-5.83), tol DCs – 4.45 (3.68-4.93), tol DCs/TNF-α – 9.70 (8.83-10.58). The intensity of HLA-DR expression on the surface of mDCs was significantly higher compared with immDCs, tolDCs and tolDCs after stimulation with TNF-α (imm DCs – 23.03 (18.24-25.40) RFI; tolDCs – 27.54 (25.04-32.41) RFI; tolDC – 10.56 (9.21-12.77) RFI; tolDC/TNF-α – 13.74 (10.66-18.53) RFI (RFI – relative fluorescence intensity).
Conclusion. Adding of the TNF-α as maturation inducer did not result in immunophenotypic reversion of tolDCs to the mDCs level. The obtained data indicate in favor of possibility to use safely tolDCs as a biomedical cell product for the treatment of diseases associated with an excessive immune response.
Supporting agencies: This research was carried out within activities of the 23rd State program “High Technologies and Technique” 2021-2025 (No. 20213421).
About the Authors
Ya. S. Minich
Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus
Belarus
Belarus
Yana S. Minich - Researcher at the Laboratory of Immunology and Virology, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
Minsk
A. Y. Hancharou
Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus
Belarus
Belarus
Andrei Y. Hancharou - Candidate of Medical Sciences, Associate Professor, Director of the Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
Minsk
N. G. Antonevich
Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus
Belarus
Belarus
Natalia G. Antonevich - Candidate of Biological Sciences, Associate Professor, Leading Researcher at the Laboratory of Immunology and Virology, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.
Minsk
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For citations:
Minich Ya.S., Hancharou A.Y., Antonevich N.G. Study of the potential of tolerogenic dendritic cells to immunophenotypic reversion affected by tumor necrosis factor. Health and Ecology Issues. 2025;22(2):69-75. (In Russ.) https://doi.org/10.51523/2708-6011.2025-22-2-08
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