ACCELERATION OF THE FIXATION OF SURGICAL AND BIOPSY MATERIAL

Abstract

Objective: to develop a method for accelerating the fixation of biopsy material by adding dimexide to formalin. Material and methods. The method of adding food coloring E124 (ponso 4R) (1 mg/ml) to buffered formalin was used to study its penetration into biopsy material by leiomyomas delivered for an urgent histological study. In the test group, dimexide was added to the structure of the biopsy material as a dye penetration accelerator at a final concentration of 20%. The material was evaluated morphometrically in percents of the area of the stained areas. Results. The complete staining of all the samples of the experimental series with the addition of dimexide was observed after 12 hours of the fixation, and in the control sample only in three pieces out of six. In the remaining three samples, 75; 76 and 86% were painted. In two observations of breast cancer the expression of the fixation with the addition of 20% dimexide concentration to formalin after 12 hours corresponded to 24 hours of the fixation in the usual way. Conclusion. The addition of dimexide to buffered formalin at a final concentration of 20% made it possible to substantially accelerate the fixation without distorting the results of IGH examining the expression of markers included in the standard panel of IHC studies (Er, Pg, Ki-67, HER2).