Antibacterial Activity of Hypogymnia Physodes Extract and its Combinations with Antibiotics Against Enterococci
https://doi.org/10.51523/2708-6011.2018-15-4-10
Abstract
Objective : to study antibacterial properties of Hypogymnia physodes extract and its combinations with antibiotics against vancomycin-sensitive and vancomycin-resistant strains of enterococci. Material and methods. Minimum inhibitory concentrations (MICs) of the acetone extract of H. physodes for reference and clinical isolates of Enterococcus faecalis were determined by the serial broth dilution method. Evaluation of efficiency of the combined effect of the H. physodes extract in combination with antibiotics was carried out using the modified disc-diffusion method and «chessboard» method. Results. We have revealed marked antibacterial activity (MIC 32-64 mcg/ml) of the acetone extract of H. physodes against vancomycin-sensitive and vancomycin-resistant strains of enterococci and found a synergistic effect (ΣFIC from 0.125 to 0.375) of the combination of the H. physodes extract and aminoglycosides against E. faecalis , including vancomycin-resistant strains. Conclusion. H. physodes is a promising source of compounds with antibacterial activity, further research is required to identify and isolate a secondary metabolite having synergistic activity in combination with aminoglycosides from the lichen extract.
About the Authors
D. V. Tapalsky
Educational institution "Gomel State Medical University"
Belarus
Belarus
K. M. Kosenkova
Educational institution "Gomel State Medical University"
Belarus
Belarus
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Review
For citations:
Tapalsky D.V., Kosenkova K.M. Antibacterial Activity of Hypogymnia Physodes Extract and its Combinations with Antibiotics Against Enterococci. Health and Ecology Issues. 2018;(4):52-56. (In Russ.) https://doi.org/10.51523/2708-6011.2018-15-4-10
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