The Results of Identification of Microorganisms from the Uterine Cavity by Means of the Method of 16s Rrna Gene Fragment Sequencing

Abstract

Objective: to identify microorganisms present in the biopsy material of the endometrium using the method of 16s rRNA gene fragment sequencing. Material and methods . Using the method of 16s rRNA gene fragment sequencing, we identified the microorganisms present in the endometrial biopsy material in 56 patients. Additionally, identification of mixed bacterial cultures obtained during subculturing of the endometrial biopsy material was carried out. Results. The sequencing method was used to determine the type of microorganisms in mixed cell culture in 18 (32.1 %) patients and in the endometrial tissue in 30 (53.6 %) patients. The predominant microorganisms in the biopsy material of the endometrium are Lactobacillus spp. , detected in 10 (33.3 %) patients and Staphylococcus spp. , revealed in 9 (30 %) patients. Based on the immunohistochemical examination, chronic endometritis was diagnosed in 26 (86.7 %) patients with positive sequencing results in the endometrial tissue. Gr+cocci dominate in patients with chronic endometritis (c² = 3.98; p = 0.05), Lactobacilli - in patients without chronic endometritis (c² = 6.35; p =0.01). The species composition of microorganisms in the cervical canal and endometrium were not identical in 17 (56.7%) cases. Conclusion. Determination of the type of microorganisms by means of the method of 16s rRNA gene fragment sequencing in the endometrial tissue is a highly informative diagnostic method and it is expedient to use it to detect inflammatory diseases of the endometrium in cases of reproductive malfunction.