Effect of cellulose when applied for tamponade of the external auditory canal on leukocyte blood count and C-reactive protein in experimental animals

Objective. To evaluate the properties of various materials used for tamponade of the external auditory canal after surgery in an animal experiment by studying the separate indices of the general blood analysis and C-reactive protein (CRP).

Materials and methods. The article presents the results of the experiment using laboratory animals for studying comparative evaluation of the properties of cellulose as a material for tamponade of the external auditory canal with a gauze tampon, and a Merocel type tampon. The total of 36 chinchilla rabbits were used in the experiment. Analysis of individual indicators of the general blood test and CRP was carried out in the postoperative period.

Results. The experiment showed that there are no differences in the number and ratio of leukocytes of different populations and subpopulations in leukocytic formula in experimental and control groups of laboratory animals. Our studies did not reveal statistically significant differences in the amount of CRP as well.

Conclusion. The experimental results indicate the potential safety and biocompatibility of cellulose when used in clinical practice. Cellulose can be considered as a promising material for making a tampon used after middle ear surgery.

1. Khamgushkeeva NN, Chernushevich II, Anikin IA, Kuzovkov VE, Dvoryanchikov VV. Middle ear packing agents. Russian Otorhinolaryngology. 2022;21(6):94-102. (In Russ.). DOI: https://doi.org/10.18692/1810-4800-2022-6-94-102

2. Mizgirev DV, Vasilieva EYu. Method the objective structured assessment of practical skills. Meditsinskoe Obrazovanie i Vuzovskaya Nauka. 2017;(2):46-50. (In Russ.).

3. Shen Y, Teh BM, Friedland PL, Eikelboom RH, Atlas MD. To Pack or Not to Pack? A Contemporary Review of Middle Ear Packing Agents. Laryngoscope. 2011;121(5):1040-1048. DOI: https://doi.org/10.1002/lary.21470

4. Gensh KV, Bazarnova NG. Oxidized cellulose. Preparation. application in medicine. Khimiya Rastitenogo Syrya. 2013;(4):13-20. (In Russ.). DOI: https://doi.org/10.14258/jcprm.1304013

5. Rybin BM, Zavrazhnova IAE, Rybin DB. Determination of physical parameters of polymers for woodworking functions on the additive group contributions chemical structural units. Lesnoy Vestnik. Forestry Bulletin. 2018;22(2):68-75. (In Russ.). DOI: https://doi.org/10.18698/2542-1468-2018-2-68-75

6. Be Miller JN. 8 - Cellulose and cellulose-based hydrocolloids. In: Be Miller JN. Carbohydrate Chemistry for Food Scientists. Amsterdam: AACCI; 2019. p. 223-240. DOI: https://doi.org/10.1016/B978-0-12-812069-9.00008-X

7. Khorov O, Sak V, Nikifarava A, Rapetskaya V. Evaluation of properties of various materials used for postoperative tamponade after ear surgery. Otorhinolaryngology. Eastern Europe. 2024;14(2):166-175. (In Russ.). DOI: https://doi.org/10.34883/PI.2024.14.2.029

8. Khorov OG, Sak VN, Chernova NN, Yanovich RV. Use of split cellulose for ear packing: comparative experimental evaluation based on postoperative clinical and microbiological observations. Folia Otorhinolaryngologiae et Pathologiae Respiratoriae. 2024;30(2):149-156. (In Russ.). DOI: https://doi.org/10.33848/fopr630416

9. Novoseletskiy VA. Experimental study of the effect of modified ultrahigh molecular weight polyethylene on some immunity parameters during its implantation in the middle ear. Military Medicine. 2013;(2):70-74. (In Russ.).

10. Astashkin EI, Achkasov EE, Berzin IA, Beskova TB, Boyarintsev VV, Vasilev AN, et al. The guide to laboratory animals and alternative models in biomedical researches. Moskow: Profil-2S; 2010. 358 p. (In Russ.).

11. Arzamascev EV, Guskova TA, Liberman SS, LjubimovBI, Rudakov AG, Verstakova OL. Methodological recommendations for studying the general toxic effect of pharmacological agents. [date of access 2024 December 18]. Available from: http://histopathology.narod.ru/documents/izuchenie_obwetoksicheskogo_dejstvie_farmasredstv.html (In Russ.).

12. Lugovskaya SA, Morozova VT, Pochtar ME, Dolgov VV. Laboratory haematology. Moscow: Triada; 2006. 223 p. (In Russ.).

13. Zakiev TZ, Tuysin SR, Gilfanov AR, Sagdiev RD, Zakieva IV. Searching for methods to predict septic complications in surgical patients. Kazan Medical Journal. 2015;96(3):294-297. (In Russ.). DOI: https://doi.org/10.17750/KMJ2015-294