Effect of the food additive E407a on E-cadherin expression in the small intestine
https://doi.org/10.51523/2708-6011.2021-18-2-14
Abstract
Objective: to assess the features of the expression of the epithelial marker E-cadherin in the epithelial layer and stroma of the small intestinal mucous membrane after the administration of the food additive E407a.
Materials and methods. E-cadherin expression was studied by the immunohistochemical method in 8 WAG rats receiving daily the food additive E407a orally for a period of 2 weeks in the amount of 140 mg per kg of body weight, and 8 control animals.
Results. It has been found that the administration of E407a leads to reduced E-cadherin expression in intestinal epithelial cells (0.037 ± 0.004 U versus 0.129 ± 0.021 U in the control group, p<0.0001) per unit of area (250 × 250 micron).> < 0.0001) per unit of area (250 × 250 micron).
Conclusion. Ingestion of the food additive E407a leads to a change in the expression patterns of the epithelial marker E-cadherin, which may contribute to the disruption of the intestinal epithelial barrier.
About the Authors
A. S. Tkachenko
Kharkiv National Medical University
Ukraine
Ukraine
Anton S. Tkachenko, PhD (Med), Associate Professor, Director of the Research Institute of Experimental and Clinical Medicine
Kharkiv
G. I. Gubina-Vakulyck
Kharkiv National Medical University
Ukraine
Ukraine
Galina I. Gubina-Vakulyck, DMedSc, Professor, Professor at the Department of Pathological Anatomy
Kharkiv
H. V. Polikarpova
Kharkiv National Medical University
Ukraine
Ukraine
Hanna V. Polikarpova, PhD (Biol), Associate Professor at the Department of Biological Chemistry
Kharkiv
A. I. Onishchenko
Kharkiv National Medical University
Ukraine
Ukraine
Anatolii I. Onishchenko, PhD (Med), Deputy Director of the Research Institute of Experimental and Clinical Medicine
Kharkiv
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Review
For citations:
Tkachenko A.S., Gubina-Vakulyck G.I., Polikarpova H.V., Onishchenko A.I. Effect of the food additive E407a on E-cadherin expression in the small intestine. Health and Ecology Issues. 2021;18(2):94-101. (In Russ.) https://doi.org/10.51523/2708-6011.2021-18-2-14
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