zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2021-18-2-14zdor-1947Research ArticleЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНА И БИОЛОГИЯEXPERIMENTAL MEDICINE AND BIOLOGYВлияние пищевой добавки Е407а на экспрессию Е-кадгерина в тонком кишечнике крысEffect of the food additive E407a on E-cadherin expression in the small intestinehttps://orcid.org/0000-0002-1029-1636ТкаченкоА. С.TkachenkoA. S.

Ткаченко Антон Сергеевич, к.м.н., доцент, директор НИИ экспериментальной и клинической медицины

Харьков

Anton S. Tkachenko, PhD (Med), Associate Professor, Director of the Research Institute of Experimental and Clinical Medicine

Kharkiv

antontkachenko555@gmail.comhttps://orcid.org/0000-0003-1207-2688Губина-ВакуликГ. И.Gubina-VakulyckG. I.

Губина-Вакулик Галина Ивановна, д.м.н., профессор, профессор кафедры патологической анатомии

Харьков

Galina I. Gubina-Vakulyck, DMedSc, Professor, Professor at the Department of Pathological Anatomy

Kharkiv

gvgipatology@gmail.comhttps://orcid.org/0000-0001-9280-9285ПоликарповаА. В.PolikarpovaH. V.

Поликарпова Анна Валериевна, к.б.н., доцент кафедры биологической химии

Харьков

Hanna V. Polikarpova, PhD (Biol), Associate Professor at the Department of Biological Chemistry

Kharkiv

h.polikarpova@yahoo.comhttps://orcid.org/0000-0002-2122-2361ОнищенкоА. И.OnishchenkoA. I.

Онищенко Анатолий Игоревич, к.м.н., заместитель директора НИИ экспериментальной и клинической медицины

Харьков

Anatolii I. Onishchenko, PhD (Med), Deputy Director of the Research Institute of Experimental and Clinical Medicine

Kharkiv

onishchenkoai@ukr.netХарьковский национальный медицинский университетKharkiv National Medical University20213006202118294101Copyright © Ткаченко А.С., Губина-Вакулик Г.И., Поликарпова А.В., Онищенко А.И., 20212021Ткаченко А.С., Губина-Вакулик Г.И., Поликарпова А.В., Онищенко А.И.Tkachenko A.S., Gubina-Vakulyck G.I., Polikarpova H.V., Onishchenko A.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/1947Цель исследования

Цель исследования: изучить особенности экспрессии эпителиального маркера Е-кадгерина в эпителиальном слое и строме слизистой оболочки тонкого кишечника при употреблении пищевой добавки Е407а.

Материалы и методы

Материалы и методы. Экспрессию Е-кадгерина изучали иммуногистохимическим методом у 8 крыс популяции WAG, получавших ежедневно в течение 2 недель пищевую добавку Е407а перорально в количестве 140 мг на кг веса, и 8 контрольных животных.

Результаты

Результаты. Установлено, что употребление Е407а приводит к снижению экспрессии Е-кадгерина в эпителиоцитах (0,037 ± 0,004 УЕОП против 0,129 ± 0,021 УЕОП у контроля, р < 0,0001) тонкого кишечника на фоне повышения количества кадгерин-положительных клеток в строме органа (5,03 ± 0,30 клеток по сравнению с 2,55 ± 0,20 клеток в контрольной группе, р < 0,0001) на единицу площади (250 × 250 мкм).

Заключение

Заключение. Пероральное употребление пищевой добавки Е407а приводит к изменению особенностей экспрессии эпителиального маркера Е-кадгерина, что может вносить вклад в нарушение эпителиального барьера кишечника.

Objective

Objective: to assess the features of the expression of the epithelial marker E-cadherin in the epithelial layer and stroma of the small intestinal mucous membrane after the administration of the food additive E407a.

Materials and methods

Materials and methods. E-cadherin expression was studied by the immunohistochemical method in 8 WAG rats receiving daily the food additive E407a orally for a period of 2 weeks in the amount of 140 mg per kg of body weight, and 8 control animals.

Results

Results. It has been found that the administration of E407a leads to reduced E-cadherin expression in intestinal epithelial cells (0.037 ± 0.004 U versus 0.129 ± 0.021 U in the control group, p<0.0001) per unit of area (250 × 250 micron).> < 0.0001) per unit of area (250 × 250 micron).

Conclusion

Conclusion. Ingestion of the food additive E407a leads to a change in the expression patterns of the epithelial marker E-cadherin, which may contribute to the disruption of the intestinal epithelial barrier.

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The authors declare that there are no conflicts of interest present.