THE METHOD OF MOLECULAR AND GENETIC DIAGNOSIS OF THE PROCESS OF CELL SENESCENCE BASED ON QUANTITATIVE ANALYSIS OF NUCLEAR AND MITOCHONDRIAL DNA GENES
https://doi.org/10.51523/2708-6011.2016-13-1-10
Abstract
Objective: to test the method of molecular and genetic diagnosis of cellular senescence based on quantitative analysis of nuclear genes (nDNA) and mitochondrial DNA (mtDNA). Material and methods. The sample of patients was 81, the material for the study - whole blood. The quantitative analysis of mtDNA was carried out using the method of polymerase chain reaction in real time (RT-PCR) protocol TaqMan. The differences of average values of the relation of mtDNA/nDNA age groups were assessed by one-way ANOVA. Results. The difference between the average value of the ratio of mtDNA / nDNA age groups is statistically significant at p < 0.05, the strength of the influence of the age factor is 46 %. Conclusion. The proven method based on the calculation of the ratio of mtDNA copy number loci (mtND1) to nuclear DNA (RPPH1) with the application of the standards can be used for the quantitative analysis of mtDNA as a genetic marker of age-related changes.
About the Authors
E. V. Voropayev
Gomel State Medical University
Belarus
Belarus
A. A. Ziatskov
Gomel State Medical University
Belarus
Belarus
O. V. Osipkina
Gomel State Medical University
Belarus
Belarus
O. Y. Baranov
Gomel State Medical University
Belarus
Belarus
N. V. Galinovskaya
Gomel State Medical University
Belarus
Belarus
V. N. Dotsenko
Gomel City Clinical Emergency Hospital
Belarus
Belarus
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Review
For citations:
Voropayev E.V., Ziatskov A.A., Osipkina O.V., Baranov O.Y., Galinovskaya N.V., Dotsenko V.N. THE METHOD OF MOLECULAR AND GENETIC DIAGNOSIS OF THE PROCESS OF CELL SENESCENCE BASED ON QUANTITATIVE ANALYSIS OF NUCLEAR AND MITOCHONDRIAL DNA GENES. Health and Ecology Issues. 2016;(1):46-50. (In Russ.) https://doi.org/10.51523/2708-6011.2016-13-1-10
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