THE COMPARATIVE ANALYSIS OF PHENOTYPING AND GENOTYPING OF N-ACETYLATION POLYMORPHISM IN HEALTHY VOLUNTEERS FROM THE SOUTH-EAST CAUCASIAN POPULATION OF BELARUS
https://doi.org/10.51523/2708-6011.2015-12-4-6
Abstract
Objective: comparative analysis of phenotyping and genotyping of N-acetylation polymorphism in healthy volunteers from the south-east region of the Republic of Belarus. Material and methods. We identified the genotype NAT2 in 30 healthy volunteers from the south-east region of the Republic of Belarus using the method PCR-RFLP by 5 mononucleotide changes (T341C, G590A, G857A, C282T, C481T). Results. It was proved that mutant alleles occurred in any N-acetyltransferase activity (p = 0.08). The number of mutant alleles of NAT2 gene showed a direct moderate association with the speed of acetylation (τ = -0.633, p < 0.0001). Probabilities for slow acetylation phenotype increases with the growing number of SNP (τ = -0.657, p < 0.0001), and the presence of 4 single nucleotide substitutions indicates a high degree of confidence for the slow acetylation phenotype (p = 0.0007). Conclusion. The simultaneous assessment of several SNPs in NAT2 gene increases the accuracy of prognosis for NAT2 acetylation phenotype, but even the simultaneous assessment of 5 SNPs does not make it possible to predict the phenotype of acetylator definitely.
Keywords
About the Authors
T. V. Satyrova
Gomel State Medical University
Belarus
Belarus
E. I. Mikhailova
Gomel State Medical University
Belarus
Belarus
O. Yu. Baranov
Gomel State Medical University
Belarus
Belarus
E. V. Voropayev
Gomel State Medical University
Belarus
Belarus
O. V. Osipkina
Gomel State Medical University
Belarus
Belarus
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Review
For citations:
Satyrova T.V., Mikhailova E.I., Baranov O.Yu., Voropayev E.V., Osipkina O.V. THE COMPARATIVE ANALYSIS OF PHENOTYPING AND GENOTYPING OF N-ACETYLATION POLYMORPHISM IN HEALTHY VOLUNTEERS FROM THE SOUTH-EAST CAUCASIAN POPULATION OF BELARUS. Health and Ecology Issues. 2015;(4):32-36. (In Russ.) https://doi.org/10.51523/2708-6011.2015-12-4-6
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