MUTATIONAL ANALYSIS OF FLT3 AND NPM1 GENES IN THE GROUP OF ADULT PATIENTS WITH MYELODYSPLASTIC SYNDROME AND ACUTE NON-LYMPHOBLASTIC LEUKEMIA

The molecular-genetic analysis of DNA samples extracted from the blood and marrow of 14 patients with myelodysplastic syndrome (refractory anemia with excess blasts) and 52 patients with acute non-lymphoblastic leukemia has been performed. It did not reveal any clinically unfavourable mutations of FLT3 gene in the group of the patients with myelodysplastic syndrome. The frequency rates for FLT3-ITD, FLT3 D835 and NPM1 mutations in the group of the patients with acute non-lymphoblastic leukemia made up 21,2 ± 5,7; 5,8 ± 3,2 and 23,1 ± 5,8 per cent, correspondingly. The subgroup of the patients with acute promyelocytic leukemia (М3) detected more FLT3-ITD mutations while the subgroup with acute myeloblastic leukemia (М1) had more NPM1 gene mutations (most of the cases in association with FLT3-ITD mutation). It was shown that a more sensitive genetic test required the use of both blood and marrow DNA samples.

миелодиспластический синдром, острый нелимфобластный лейкоз, FLT3-ITD, FLT3 D835, NPM1, полимеразная цепная реакция, myelodysplastic syndrome, acute non-lymphoblastic leukemia, FLT3-ITD, FLT3 D835, NPM1, polymerase chain reaction

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