Structural diversity of erythrocytes in patients with hereditary spherocytosis
https://doi.org/10.51523/2708-6011.2018-15-2-22
Abstract
Objective: to study the shape of erythrocytes and structure of their surface layer including the membrane and cytoskeleton (actin-spectrin network) in child patients with hereditary spherocytosis. Material and methods. The methods of optic and atomic-force microscopy were used in the study. Results. A variety of erythrocyte shapes with such prevalent types as discocytes, spherocytes, and echinocytes were revealed in the blood of the patients. The surface of certain cells contained microvesicules. The spatial heterogeneity of the structure of mechanical property maps of the cell surface layer was detected. Conclusion. The diversity of erythrocyte features in patients with hereditary spherocytosis is present both at the level of the cell shapes and at the level of the structure of mechanical property maps of their surface layer.
About the Authors
M. N. Starodubtseva
Gomel State Medical University
Belarus
Belarus
E. F. Mitsura
Republican Research Center for Radiation Medicine and Human Ecology
Belarus
Belarus
I. A. Chelnokova
Gomel State Medical University
Belarus
Belarus
A. N. Kondrachuk
Gomel State Medical University
Belarus
Belarus
N. I. Yegorenkov
Gomel State Medical University
Belarus
Belarus
References
1. Perrotta S, Gallagher PG, Mohandas N. Hereditary spherocytosis. Lancet. 2008Oct;372(9647):1411-26.
2. Eber S, Lux SE. Hereditary spherocytosis--defects in proteins that connect themembrane skeleton to the lipid bilayer. Semin Hematol. 2004 Apr;41(2):118-41.
3. Barcellini W, Bianchi P, Fermo E, Imperiali FG, Marcello AP, Vercellati C, et al. Hereditary red cell membrane defects: diagnostic andclinical aspects. Blood Transfus. 2011 Jul;9(3):274-7.
4. Mahajan V, Jain SK. Hereditary Spherocytosis. NeoReviews. 2016 Dec;17 (12) e697-e704.
5. King MJ, Garcon L, Hoyer JD, Iolascon A, Picard V, Stewart G, et al. International Council for Standardization in Haematology. ICSH guidelines for the laboratory diagnosis of nonimmune hereditary red cell membranedisorders. Int J Lab Hematol. 2015 Jun;37(3):304-25.
6. Gallagher PG. Abnormalities of the erythrocyte membrane. Pediatr Clin North Am. 2013 Dec;60(6):1349-62.
7. Christensen R, Yaish H, Henry E, Baer V, Bennett S. A simple method of screening newborn infants for hereditary spherocytosis. J Appl Hematol. 2013 Jun;4:27-32.
8. Andolfo I, Russo R, Gambale A, Iolascon A. New insights on hereditary erythrocyte membrane defects. Haematologica. 2016 Oct; 101(11):1284-1294.
9. Lux SE 4th. Anatomy of the red cell membrane skeleton: unanswered questions. Blood. 2016 Jan 14;127(2):187-99.
Review
For citations:
Starodubtseva M.N., Mitsura E.F., Chelnokova I.A., Kondrachuk A.N., Yegorenkov N.I. Structural diversity of erythrocytes in patients with hereditary spherocytosis. Health and Ecology Issues. 2018;(2):109-114. (In Russ.) https://doi.org/10.51523/2708-6011.2018-15-2-22
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