THE TECHNIQUE OF MAKING AND STAINING OF FILM PREPARATIONS (FRAGMENTS) CONSISTING OF FRAMED DENSE FIBROUS CONNECTION TISSUE

Abstract

Objective: to develop a histochemical staining technique that allows to make a more complete evaluation of the morphological characteristics of both separate cells and tissue structures in comparison with other methods. Materials. Data from sectional examination of 70 subjects: 35 subjects of the control group (staining of ligament fragments in this group was carried out with hematoxylin-eosin) and 35 subjects of the main group (staining of ligament fragments in this group was performed with toluidine blue and nuclear strong red). Results. The range of values of the nuclear-cytoplasmic ratio when material was stained with toluidine blue and nuclear fast red was much narrower and contained less «emissions» than the range of values of the nuclear-cytoplasmic ratio when stained with hematoxylin-eosin: 0.46 ± 0.04 and 0.46 ± 0.07, respectively. Conclusion. The proposed new staining method makes it possible to get the preparation quickly (the time from the moment of taking the material till procurement of the finished preparation is about 3 hours), and besides, it ensures obtainment of data with a smaller spread of the values of the nuclear-cytoplasmic ratio in comparison with the traditional staining technique.