Influence of pooling mesenchymal stem cells on the suppression of proliferative activity of T-lymphocytes
https://doi.org/10.51523/2708-6011.2024-21-2-12
Abstract
Objective. To determine the effect of pooling (merging) of single cultures of olfactory mucosa-derived mesenchymal stem cells (MSCs) of the nasal cavity on the suppression of the proliferative activity of T-lymphocytes.
Materials and methods. Using flow cytometry, the effect of pooling single cultures of MSCs obtained from healthy donors (n=7) on the mitogen-induced proliferation of T-lymphocytes in the peripheral blood of volunteers (n=5) was studied. The proliferation index and the number of dividing CD3+ T-cells were assessed by changes in the fluorescence intensity of Tag-it Vio. Statistical processing of the obtained data was carried out using non-parametric statistics.
Results. An evaluation of 35 variants of co-culture of single MSCs with T-cells and 33 variants of co-culture of pooled MSCs with T-cells was performed. The inhibitory effect of both single (p = 0.0001) and pooled MSCs (p = 0.0001) on
PHA-induced proliferation of CD3+ T-cells in the total fraction of peripheral blood lymphocytes was identified. It was shown that pooled MSCs have a significantly more pronounced suppressive effect on T-cell proliferation compared to single MSCs (p=0.000004).
Conclusion. PoolMSCs have a significantly more pronounced effect in suppressing PHA-induced proliferation of peripheral blood T-cells compared to MSC monocultures, which justifies the use of poolMSCs in medicine to achieve better results in the treatment of immune-mediated diseases.
About the Authors
A. H. Rynda
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Alena H. Rynda, Postgraduate Student at the Laboratory of Immunology and Virology
Minsk
A. Y. Hancharou
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Andrei Y. Hancharou, Candidate of Medical Sciences, Associate Professor, Director
Minsk
N. G. Antonevich
Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus
Belarus
Belarus
Natalia G. Antonevich, Candidate of Biological Sciences, Leading Researcher
Minsk
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For citations:
Rynda A.H., Hancharou A.Y., Antonevich N.G. Influence of pooling mesenchymal stem cells on the suppression of proliferative activity of T-lymphocytes. Health and Ecology Issues. 2024;21(2):97-102. (In Russ.) https://doi.org/10.51523/2708-6011.2024-21-2-12
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