Study of the sensitivity of methicillin-resistant Staphylococcus aureus to antibiotics and bacteriophage preparations
https://doi.org/10.51523/2708-6011.2024-21-1-12
Abstract
Objective. To evaluate the sensitivity of methicillin-resistant Staphylococcus aureus strains to antibiotics and therapeutic and prophylactic preparations of bacteriophages.
Materials and methods. The antibiotic sensitivity of strains of methicillin-resistant S.aureus was studied by the disco-diffusion method, the determination of the minimum inhibitory concentrations of vancomycin and linezolid by broth microdilution method, and ceftaroline by E-tests. The sensitivity of methicillin-resistant S.aureus to bacteriophage preparations was studied.
Results. It has been established that linezolid and vancomycin are quite active drugs against methicillin-resistant S.aureus (MRSA), however, resistance to them begins to develop. MRSA resistance to fluoroquinolones and cefoxitin was the highest of all antibiotics tested; the results obtained with cefoxitin can be interpreted for other cephalosporins (except ceftaroline) and all penicillins. Only 67.9% of MRSA strains retained sensitivity to ceftaroline, but ceftaroline-resistant strains had low levels of minimal inhibitory concentration. The preparations of the bacteriophages “Sextaphage” and “Piobacteriophage Phagio” showed their lytic activity only in relation to 35.8% and 18.9% of MRSA isolates, respectively.
Conclusion. Monitoring the development of antibiotic resistance in methicillin-resistant S.aureus is an important part of the strategy of rational antibiotic therapy for staphylococcal infections. Only taking into account the preliminary step-by-step determination of the phage sensitivity of the causative agent of the disease, bacteriophage preparations can be considered as a completely possible alternative to antibiotics for the treatment of purulent inflammatory diseases caused by MRSA.
Supporting agencies: The work was carried out without sponsorship
About the Authors
L. V. Lagun
Gomel State Medical University
Belarus
Belarus
Ludmila V. Lagun, Candidate of Medical Sciences, Associate Professor
Department of Microbiology, Virology and Immunology
Gomel
Ya. A. Kulvinsky
Gomel State Medical University
Belarus
Belarus
Yauheni A. Kulvinsky, Student
Faculty of General Medicine
Gomel
N. A. Kulvinskaya
Gomel State Medical University
Belarus
Belarus
Natallia A. Kulvinskaya, Student
Faculty of General Medicine
Gomel
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Review
For citations:
Lagun L.V., Kulvinsky Ya.A., Kulvinskaya N.A. Study of the sensitivity of methicillin-resistant Staphylococcus aureus to antibiotics and bacteriophage preparations. Health and Ecology Issues. 2024;21(1):93-101. (In Russ.) https://doi.org/10.51523/2708-6011.2024-21-1-12
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