Changes in energy metabolism of rat myocardium during exposure to ionizing radiation
https://doi.org/10.51523/2708-6011.2024-21-1-11
Abstract
Objective. To study the effect of incorporated 137Cs on the energy metabolism of the myocardium of white rats.
Materials and methods. The work was carried out on 8 male white rats divided into 2 experimental groups, which were fed products contaminated with 137Cs for 7 days. Polarography was used to study the rate of oxygen consumption by myocardial tissue preparations on endogenous substrates, with the addition of exogenous succinic and glutamic acids, and with the addition of an uncoupler of oxidative phosphorylation, 2,4-dinitrophenol.
Results. An increase in the rate of respiration on endogenous substrates caused by the uncoupling of oxidative phosphorylation was noted. At the same time, no statistically significant change in the amount of endogenous substrates was found.
Conclusion. The revealed violation of energy production in the myocardium of experimental animals is caused by the uncoupling of oxidative phosphorylation, and can be explained by a change in the operation of potassium channels, which can explain the development of cardiovascular pathology under the action of ionizing radiation.
Supporting agencies: The study was conducted without sponsorship
About the Author
A. N. Koval
Gomel State Medical University
Belarus
Belarus
Alexander N. Koval, Candidate of Biological Sciences., Associate Professor
Department of Biological Chemistry
Gomel
References
1. Stewart JR, Fajardo LF. Radiation-induced heart disease: an update. Progress in Cardiovascular Diseases. 1984;27(3):173-194. doi: 10.1016/0033-0620(84)90003-3
2. Gritsuk AI, Koval AN, Gritsuk NA. Ionizing radiation as a risk factor for the development of cardiovascular diseases. Radiobiology: minimizing radiation risks : Proceedings of the International scientific conference, Gomel, September 29–30, 2016. Gomel: Institute of Radiology. 2016;63-66. [date of access 2022 September 20]. Available from: https://www.elibrary.ru/wlxmfl
3. Hausenloy DJ, Schulz R, Girao H, et al. Mitochondrial ion channels as targets for cardioprotection. Journal of Cellular and Molecular Medicine. 2020;24(13). doi: 10.1111/jcmm.15341
4. Kulawiak B, Bednarczyk P, Szewczyk A. Multidimensional Regulation of Cardiac Mitochondrial Potassium Channels. Cells. 2021;10(6):1554. Published 2021 Jun 19. doi: 10.3390/cells10061554
5. Wester PO. Concentration of 17 elements in subcellular fractions of beef heart tissue determined by neutron activation analysis. Biochem. Biophys. Acta. 1965;109(1):268-283. doi: 10.1016/0926-6585(65)90111-1
6. Li C, Chen H, Yang X, Wang K, Liu J. An ion transport switch based on light-responsive conformation-dependent G-quadruplex transmembrane channels. Chem Commun (Camb). 2021;57(66):8214-8217. doi: 10.1039/d1cc03273a
7. Jana J, Vianney YM, Schröder N, Weisz K. Guiding the folding of G-quadruplexes through loop residue interactions. Nucleic Acids Research. 2022;50(12):7161-7175. doi: 10.1093/nar/gkac549
8. Balasubramanian S, Hurley LH, Neidle S. Targeting G-quadruplexes in gene promoters: a novel anticancer strategy? Nature Reviews Drug Discovery. 2011;10(4):261-275. doi: 10.1038/nrd3428
9. Vianney YM, Weisz K. First Tandem Repeat of a Potassium Channel KCNN4 Minisatellite Folds into a V-Loop G-Quadruplex Structure. Biochemistry. 2021;60(17):1337-1346. doi: 10.1021/acs.biochem.1c00043
10. Gritsuk AI, Koval AN. The role of guanine quadruplexes as a possible target for influencing mitochondrial DNA during 137Cs incorporation. Radiobiology: actual problems. Mater. Intl. scientific conference (September 27-28, 2018). Gomel, 2018;50-53.
11. Moiseev AA., Ivanov VI. Reference book on dosimetry and radiation hygiene. М.;1990. 252 p.
12. Gritsuk AI, Werner AI, Matyukhina TG, et al. Effect of incorporated cesium radionuclides on ultrastructure and processes of tissue respiration of mitochondria of cardiomyocytes. Proceedings of the National Academy of Sciences of Belarus, Medical series. 2023;20(1):42-57. 2002;(2):63-70.
Review
For citations:
Koval A.N. Changes in energy metabolism of rat myocardium during exposure to ionizing radiation. Health and Ecology Issues. 2024;21(1):89-92. (In Russ.) https://doi.org/10.51523/2708-6011.2024-21-1-11
Views: 207
Email this article 