Platelet-associated NETosis in monitoring inflammation in rheumatoid arthritis and systemic lupus erythematosus
https://doi.org/10.51523/2708-6011.2023-20-4-11
Abstract
Objective. To assess the possibility of using platelet-associated NETosis parameters to monitor inflammatory activity in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE).
Materials and methods. 50 patients with RA and 29 patients with SLE were examined. The parameters of early and late platelet-associated NETosis were assessed in autologous mixed cultures containing a leukocyte suspension and intact and ADP-stimulated platelets.
Results. In patients with RA and SLE in the acute stage, rates of platelet-associated NETosis were increased compared to healthy individuals (p = 0,00001-0,001). During the period of clinical remission, NETosis parameters decreased in comparison with the exacerbation stage (NET-Рst: p=0,017-0,04), in some cases reaching control values. Based on regression analysis, threshold values for platelet-associated NETosis parameters were determined to assess the activity of the inflammatory process in patients with RA and SLE.
Conclusion. Parameters of platelet-associated NETosis are informative laboratory markers of the inflammatory process in patients with RA and SLE.
About the Author
Zh. V. Zubkova
Gomel State Medical University
Belarus
Belarus
Zhanna V. Zubkova, Senior Lecturer at the Department of Clinical Laboratory Diagnostics, Allergology and Immunology
Gomel
References
1. Zinchuk IYu, Amirdzhanova VN. Social burden of rheumatoid arthritis. Rheumatology Science and Practice. 2014;52(3):331-335 (In Russ.). DOI: https://doi.org/10.14412/1995-4484-2014-331-335
2. Panafidina TA, Popkova TV, Aseeva EA, Lila AM. A Modern Approach to the Diagnosis and Management of Systemic Lupus Erythematosus. Doctor.Ru. 2021;20(7):40-50. (in Russ.). DOI: https://doi.org/10.31550/1727-2378-2021-20-7-40-50
3. Nasonov EL, Olyunin YuA, Lila AM. Rheumatoid arthritis: the problems of remission and therapy resistance. Nauchno-Prakticheskaya Revmatologiya= Rheumatology Science and Practice. 2018;56(3):363-271 (In Russ.). DOI: https://doi.org/10.14412/1995-4484-2018-263-271
4. Kaplan MJ Role of neutrophils in systemic autoimmune diseases. Arthritis Res Ther. 2013 2013, 15:219. DOI: https://doi.org/10.1186/ar4325
5. Serebryanaya NB, Shanin SN, Fomicheva EE, Yakutseni PP. Blood platelets as activators and regulators of inflammatory and immune reactions. Part 2. Thrombocytes as participants of immune reactions. Medical Immunology. 2019;21(1):9-20. (in Russ.). DOI: https://doi.org/10.15789/1563-0625-2019-1-9-20
6. Pinegin B, Vorobjeva N, Pinegin V. Neutrophil extracellular traps and their role in the development of chronic inflammation and autoimmunity. Autoimmun. Rev. 2015;14(7):633-640.
7. Tsokos GC. Autoimmunity and organ damage in systemic lupus erythematosus. Nat Immunol. 2020;21(6):605-614. DOI: https://doi.org/10.1038/s41590-020-0677-6
8. O’Neil LJ, Kaplan MJ, Carmona-Rivera C. The role of neutrophils and neutrophil extracellular traps in vascular damage in systemic lupus erythematosus. J Clin Med. 2019;8:1325.
9. Dolgushin II, Shishkova YuS, Savochkina AYu. Technologies for determining and the role of neutrophil extracellular traps in antimicrobial protection. Vestnik RAMS. 2010;(4):26-30. (in Russ).
10. Zubkova ZhV. Method for assessing the influence of platelets on the formation neutrophil extracellular traps. In: Problems and prospects for the development of modern medicine: a collection of scientific articles of the VIII Republican Scientific and Practical Conference with the international participation of students and young scientists; 2016, April 28; Gomel. Gomel: GomSMU; 2016. pp. 269-271. [date of access 2023 October 18]. Available from: https://elib.gsmu.by/handle/GomSMU/10302
Review
For citations:
Zubkova Zh.V. Platelet-associated NETosis in monitoring inflammation in rheumatoid arthritis and systemic lupus erythematosus. Health and Ecology Issues. 2023;20(4):87–93. (In Russ.) https://doi.org/10.51523/2708-6011.2023-20-4-11
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