Changes in the level of endogenous respiration of the small intestine mucosa at various times after irradiation
https://doi.org/10.51523/2708-6011.2023-20-2-10
Abstract
Objective. To study the level of endogenous respiration of the small intestine mucosa of experimental animals after exposure to external irradiation at a dose of 0.5 and 1.0 Gy.
Materials and methods. The study was conducted on white male outbred rats weighing 180-200 g. The animals were irradiated once in the “IGUR-1” unit, 137Cs source, dose rate 0.92 Gy/min. The parameters of tissue respiration were studied by polarographic method using Record-4 device (RF) in a 2 ml cell with a closed platinum Clark electrode at 25°C.
Results. It was shown that changes in respiratory activity over time are non-monotonous. This confirms the high sensitivity of the small intestine to radiation exposure.
Conclusion. The level of endogenous respiration of the small intestine mucosa can be one of the criteria for assessing the energy status of the tissue after irradiation.
About the Author
N. S. Myshkavets
Gomel State Medical University
Belarus
Belarus
Nadeja S. Myshkavets, Lecturer at the Department of Biological Chemistry
Gomel
References
1. Khizhnyak SV, Stepanova LI, Grubskaya LV, Wojcicki VM. Functional state of the electron transfer chain of mitochondria of rat small intestine enterocytes after general external exposure to low-dose ionizing radiation. Radiation biology. Radioecology. 2013;53(6):592-597. (In Russ.).
2. Zhirnov VV, Khyzhnyak SV, Voitsitskiy VM. The effects of ultra-low dose β-radiation on the physical properties of human erythrocyte membranes. International Journal of Radiation Biology. 2010;86(6):499-506. DOI: https://doi.org/10.3109/09553001003717167
3. Khizhnyak SV, Prokhorova AA, Stepanova LI, Wojcicki VM. Functioning of the antioxidant system in the epithelial cells of the small intestine under the action of ionizing radiation with a low dose rate. Radiation biology. Radioecology. 2011;51(6):684-688. (In Russ.).
4. Gritsuk AI, Mrochek AG. Caesium, mitochondria and problems of cardiology. Vesci NAS Belarussi. Ser. med. navuk. 2008;(4):63-76. (In Russ.).
5. Gritsuk NA. Mitochondrial oxidation in cardiomyocytes and electrocardiographic parameters in rats during incorporation of 137Cs. Vesci NAS Belarussi. Ser. med. navuk. 2009;(3):63-67. (In Russ.).
6. Yaskova NS. Changes in the energy metabolism of the small intestine on the tenth day after gamma irradiation. Health and Ecology Issues. 2007;(4):141-145. (In Russ.). DOI: https://doi.org/10.51523/2708-6011.2007-4-4-31
7. Dröge W. Free radicals in the physiological control of cell function. Physiol Rev. 2002 Jan;82(1):47-95. DOI: https://doi.org/10.1152/physrev.00018.2001
8. Frank GM. Guidelines for the study of biological oxidation by polarographic method. Moscow, RF: Nauka; 1973. (In Russ.).
9. Chwalinski S, Potten CS. Radiation-induced mitotic delay: duration, dose and cell position dependence in the crypts of the small intestine in the mouse. Int J RadiatBiolRelat Stud Phys Chem Med. 1986 May; 49(5):809-819. DOI: https://doi.org/10.1080/09553008514553011
10. Ugolev AM, Zaripov BZ, Jesuitova NN, Gruzdkov AA, Rybin IS, Voloshenovich MI, et al. A revision of current data and views on membrane hydrolysis and transport in the mammalian small intestine based on a comparison of techniques of chronic and acute experiments: Experimental re-investigation and critical review. Comparative Biochemistry and Physiology. Part A: Physiology. 1986;85(4):593-612. DOI: https://doi.org/10.1016/0300-9629(86)90269-0
11. Мохова Е.Н. Дыхание митохондрий в тканевых препаратах. Регуляция энергетического обмена и физиологическое состояние организма. Москва, РФ: Наука;1978. Mokhova EN. Mitochondrial respiration in tissue preparations. Regulation of energy metabolism and the physiological state of the body. Moscow, RF: Nauka;1978. (In Russ).
12. Martin K, Potten CS, Roberts SA, Kirkwood TB. Altered stem cell regeneration in irradiated intestinal crypts of senescent mice. J CellSci. 1998 Aug;111(16):2297-2303. DOI: https://doi.org/10.1242/jcs.111.16.2297
13. Potten CS, Owen G, Roberts SA. The temporal and spatial changes in cell proliferation within the irradiated crypts of the murine small intestine. Int J RadiatBiol. 1990 Jan;57(1):185-199. DOI: https://doi.org/10.1080/09553009014550431
14. Lindsay KJ, Coates PJ, Lorimore SA, Wright EG. The genetic basis of tissue responses to ionizing radiation. Br J Radiol. 2007 Sep;80(1):2-6. DOI:https://doi.org/10.1259/bjr/60507340
15. Potten CS, Wilson JW, Booth C. Regulation and significance of apoptosis in the stem cells of the gastrointestinal epithelium. StemCells. 1997;15(2):82-93. DOI: https://doi.org/10.1002/stem.150082
Review
For citations:
Myshkavets N.S. Changes in the level of endogenous respiration of the small intestine mucosa at various times after irradiation. Health and Ecology Issues. 2023;20(2):72-77. (In Russ.) https://doi.org/10.51523/2708-6011.2023-20-2-10
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