Frequency of occurrence of the PPAR family gene polymorphisms and their relationship with lipid levels in healthy young individuals
https://doi.org/10.51523/2708-6011.2022-19-3-04
Abstract
Objective. To determine the frequency of occurrence of the genotypes of the PPARG (Pro12Ala), PPARD (294T/C), and PPARA (G2528C) gene polymorphic variants, and to establish their relationship with blood lipid levels in healthy young individuals.
Materials and methods. The study included healthy young individuals (n=90) aged 20.1 ± 0.6 years. The parameters of the lipidogram were evaluated: total cholesterol (TC), low-density lipoprotein cholesterol (LDL cholesterol), highdensity lipoprotein cholesterol (HDL cholesterol), triglycerides (TGs), atherogenicity coefficient (CA). The polymorphic variants of the PPAR genes were determined. The results of the study were processed using the STATISTICA 10.0 application package.
Results. The genotype distribution of the Pro12Ala polymorphic variant of the PPARG gene (χ2=0.0079; p=0.73), 294T/C of the PPARD gene (χ2=0.26; p=0.65), and G2528C of the PPARA gene (χ2=4.72; p=0.8) in the healthy young individuals corresponded to the expected Hardy-Weinberg equilibrium. No significant differences between the lipid levels in carriers of different variants of the polymorphic PPARG gene (Pro12A1a) were obtained.
Conclusion. Individuals with the C/C polymorphic variant had significantly higher TG levels, lower HDL cholesterol levels in the blood, and the highest CA compared with carriers of the T/T and T/C variants of the PPARD (294T/C) polymorphic gene. Carriers of the C/C polymorphic variant had significantly (p=0.049) higher LDL-C levels than carriers of the G/C variant of the PPARA (G2528C) polymorphic gene. The risk of hypertriglyceridemia is 9.6 times, of low HDL-C is 6.9 times, and of having an elevated CA rate is 5.2 times as high in individuals with the C/C polymorphic variant as in individuals with the T variants /T and T/C of the PPARD gene (294T/C).
About the Authors
Y. I. Belous
Grodno State Medical University
Belarus
Belarus
Yulia I. Belous, Senior Lecturer at the Department of General Medical Practice and Polyclinic Therapy
Grodno
L. V. Yakubava
Grodno State Medical University
Belarus
Belarus
Liudmila V. Yakubava, DMedSc, Professor, Head of the Department of General Medical Practice and Polyclinic Therapy
Grodno
Z. V. Lovkis
Scientific-Practical Center for Foodstuffs of the National Academy of Sciences of Belarus
Belarus
Belarus
Zenon V. Lovkis, DTechSc, Professor, Chief Researcher
Minsk
H. M. Morgunova
Scientific-Practical Center for Foodstuffs of the National Academy of Sciences of Belarus
Belarus
Belarus
Helena M. Morgunova, PhD (Tech), Associate Professor, Deputy Director General for Standardization and Quality of Food
Minsk
References
1. National Statistical Committee of the Republic of Belarus. Demographic. Yearbook of the Republic of Belarus. Statistical Book [Electronic resource]. Minsk, 2019. [Date of access 2021 November 18]. Available from: https://www.belstat.gov.by/upload/iblock/145/145cac172f7bf1a9801c64e20888661f.pdf (In Russ.).
2. Belous YuI, Yakubova LV, Kezhun LV, Lovkis ZV, Morgunova EM. Changes in blood lipid levels in young healthy volunteers with differentiated consumption of palm oil. Medical business. 2020;3:51-55. (In Russ.).
3. Prevalence of risk factors for non-communicable diseases in the Republic of Belarus, 2017. [Electronic resource]. STEPS 2016 (2017) WHO. [Date of access 2021 November 23]. Available from: http://www.drogcge.by/e/97049-pasprostranennost-faktorov-riska-neinfekts. (In Russ.).
4. Paradine NA, Shuvalova NV. PPAR Target receptors for drugs. The journal «Healthcare of Chuvashia». I.N. Ulyanov Chuvash State University, Cheboksary. 2012;(2). (In Russ.).
5. Racine MS, Kaidashev MS, Racine IP, Racine AM. Receptors activating peroxisome proliferation: their role in atherogenesis and development of arterial hypertension. Ukrainian Cardiology Journal. 2006; (4):106-113. (In Russ.).
6. Chen K, Chang S, Huang H., Lin T., Wu Y, Chen C. Threein-one agonists for PPAR-α, PPAR-γ, and PPAR-δ from traditional Chinese medicine. J Biomol Struct Dyn. 2012;30(6):662-683. DOI: https://doi.org/10.1080/07391102.2012.689699
7. Yang XY, Wang LH, Farrar WL. A Role for PPAR gamma in the Regulation of Cytokines in Immune Cells and Cancer. PPAR Res. 2008. DOI: https://doi.org/10.1155/2008/961753.
8. Tarkovskaya IV, Glotov OS, Ivashchenko TE, Baranov VS. Features of polymorphism of PGC-1 energy metabolism genes, PPAR and UCP families in two age groups of St. Petersburg population. Ecological genetics. 2011;9(4):35-42.
9. Chandra M, Miriyala S, Panchatcharam M. PPARγ and Its Role in Cardiovascular Diseases. PPAR Res. 2017. (In Russ.). DOI: https://doi.org/10.17816/ecogen9435-42
10. Zhijun W, Yuqing L, Wei J, Yan L, Lin L, Guoping LuThe Pro12Ala Polymorphism in the Peroxisome ProliferatorActivated Receptor Gamma-2 Gene (PPARγ2) Is Associated with Increased Risk of Coronary Artery Disease: A Meta-Analysis. PLoS ONE. 2012;7:1-14.
11. Tai ES, Corella D, Deurenberg-Yap M, Adiconis X, Chew SK, Tan CE et al. Differential effects of the C1431T and Pro12Ala PPAR gene variants on plasma lipids and diabetes risk in an Asian population. The Journal of Lipid Research. 2004;45(4):674-685. DOI: https://doi.org/10.1194/jlr.M300363-JLR200
12. Hamada T, Kotani K, Tsuzaki K, Sano Y, Murata T, Tabata M, et al. Association of Pro12Ala polymorphism in the peroxisome proliferator–activated receptor γ2 gene with small dense lowdensity lipoprotein in the general population. Metabolism. 2007;56(10):1345-1349.
13. Luo Ch, Liu Ch-W, Ge L, Pang G-F, Yang M, Hu C-Y, et al. PPARD + 294 C overrepresentation in general and long - lived population in China Bama longevity area and unique relationships between PPARD + 294T. Journal Lipids in Health and Disease. 2015;14:17. DOI: https://doi.org/10.1186/s12944-015-0016-3
14. Skogsberg J, McMahon AD, Karpe F, Hamsten A, Packard CJ, Ehrenborg E, et al. Peroxisome proliferator activated receptor delta genotype in relation to cardiovascular risk factors and risk of coronary heart disease in hypercholesterolaemic men. Journal of Internal Medicine. 2003; 254: 597-604.
15. Yılmaz-Aydogan H, Kucukhuseyin O, Kurnaz O, AkadamTeker B, Kurt O, Tekeli A, et al. Investigation of polymorphic variants of PPARD and APOE genes in Turkish coronary heart disease patients. DNA Cell Biol. 2012;31(5):867-875. DOI: https://doi.org/10.1089/dna.2011.1464
16. Akhmetov AI. Molecular genetics of sports: a monograph. Soviet sport. Moscow, 2009. 268 p. (In Russ.).
17. Doney ASF, Fischer B, Lee SP, Morris AD, Leese G, Palmer CNA. Association of common variation in the PPARA gene with incident myocardial infarction in individuals with type 2 diabetes: A Go-DARTS study. Nuclear Receptor. 2005;3:4-11.
18. Rebrova OYU. Statistical analysis of medical data. Application of the STATISTICA application software package. Moscow: Mediasphere, 2000. 312 p. (In Russ.).
Review
For citations:
Belous Y.I., Yakubava L.V., Lovkis Z.V., Morgunova H.M. Frequency of occurrence of the PPAR family gene polymorphisms and their relationship with lipid levels in healthy young individuals. Health and Ecology Issues. 2022;19(3):32-38. (In Russ.) https://doi.org/10.51523/2708-6011.2022-19-3-04
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