A radiation-induced model of dystrophic changes in the myotendinous junction in biological models: a pilot study
https://doi.org/10.51523/2708-6011.2022-19-2-11
Abstract
Objective. To evaluate the possibility of using X-ray radiation to model dystrophic lesions of the myotendinous junction in biological models (rats).
Materials and methods. The objects of the study were model animals (6 rats). The animals were irradiated in the 10 Gy and 20 Gy modes using the biological irradiation unit X-RAD 320 Precision X-ray Inc in doses of 10 and 20 Gy. The morphological examination of the hind limbs of the rats after exposure to ionizing radiation was carried out according to the standard scheme. Sonography was performed on an expert class Mindrey DС-80 ultrasound scanner (a linear transducer with an operating frequency of 18-20 MHz was used).
Results. А parallelism has been found between histological, immunohistochemical and sonographic changes in radiation-induced damage to the myotendinous junction (these changes are short-term in nature in the tendon part and long-term in the muscle part, while the severity of the changes is predetermined by the absorbed dose, which makes it possible to reproduce any stages of the pathological continuum).
Conclusion. Тhe proposed model of radiation-induced damage to the myotendinous junction allows reproducing a histological and sonographic pattern corresponding to the subacute and chronic phase of the specified pathological process without traumatic effects on the muscle and tendon.
Supporting agencies: The work was carried out within the framework of the State Program of Scientific Research for 2021–2025 "Natural Resources and the Environment", subprogram 3 "Radiation and Biological Systems" within the framework of the task "To develop criteria for the evaluation of radiation-induced changes of the tissue of the internal medium based on the analysis of the structure and mechanical features of the cell component on models in vitro and in vivo".
About the Authors
А. М. Yurkovskiy
Gomel State Medical University
Belarus
Belarus
Аlexei М. Yurkovskiy, PhD (Med), Head of the Department of Radiation Diagnostics, Radiation Therapy with the course of the Faculty of Professional Development and Retraining
Gomel
I. V. Nazarenko
Gomel State Medical University
Belarus
Belarus
Irina V. Nazarenko, PhD (Med), Associate Professor, Associate Professor at the Department of Radiation Diagnostics, Radiation Therapy with the course of the Faculty of Professional Development and Retraining
Gomel
S. L. Achinovich
Gomel Regional Clinical Oncological Dispensary
Belarus
Belarus
Sergei L. Achinovich, PhD (Med), Head of the Pathoanatomical Department
Gomel
N. N. Veyalkina
Institute of Radiobiology of the National Academy of Sciences of Belarus
Belarus
Belarus
Natalia N. Veyalkina, PhD (Biol), Head of the Laboratory of Experimental Biological Models
Gomel
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Review
For citations:
Yurkovskiy А.М., Nazarenko I.V., Achinovich S.L., Veyalkina N.N. A radiation-induced model of dystrophic changes in the myotendinous junction in biological models: a pilot study. Health and Ecology Issues. 2022;19(2):90-98. (In Russ.) https://doi.org/10.51523/2708-6011.2022-19-2-11
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