Comparison of different DNA detection options for TTV, TTMDV, and TTMV viruses
https://doi.org/10.51523/2708-6011.2022-19-1-13
Abstract
Objective. To compare different variants of TTV, TTMDV, and TTMV DNA detection in the blood plasma of patients with various liver diseases and in individuals without signs of liver disease.
Materials and methods. To detect TTV TTMDV, and TTMV DNA, the PCR method was used.
Results. A fairly high frequency of TTV virus DNA detection was found using different laboratory approaches. The frequency of TTV DNA detection was significantly highest when using the primers for the non-coding region UTR – 77.3 % compared with the coding region ORF1 – 38.4 % (p < 0.001) and the commercial kit 53 % (p < 0.005).
Conclusion. TTV DNA is detected significantly more often in patients with liver diseases compared with healthy individuals (90.3 % and 65.6 % in the UTR region and using the commercial kit, respectively), TTMV DNA (83.9 % – UTR region) and mixed DNA of the TTV + TTMDV + TTMV viruses (62.4 % – UTR region). To standardize the DNA detection of the TTV family viruses by the PCR method, it is advisable to create a panel of sera containing reliably positive and negative samples.
About the Authors
O. V. OsipkinaBelarus
Olga V. Osipkina, Head of the Research Laboratory
Gomel
E. V. Voropaev
Belarus
Evgenii V. Voropaev, PhD (Med), Associate Professor, Vice-Rector in charge of scientific work
Gomel
V. M. Mitsura
Belarus
Viktor M. Mitsura, DMedSc, Associate Professor, Deputy Director for Research, Republican Research Center for Radiation Medicine and Human Ecology; Professor at the Department of Infectious Diseases, Gomel State Medical University
Gomel
D. V. Tereshkov
Belarus
Dmitriy V. Tereshkov, Head of the Infectious Diseases Department
Gomel
A. A. Kovalev
Belarus
Alexey A. Kovalev, software engineer of the Department of Science and Scientific and Methodical Information
Gomel
References
1. Kowarsky M, Camunas-Soler J, Kertesz M, De Vlaminck I, Koh W, Pan W, et al. Numerous uncharacterized and highly divergent microbes which colonize humans are revealed by circulating cell-free DNA. Proceedings of the National Academy of Sciences of the United States of America. 2017;114(36):9623-9628. https://doi.org/10.1073/pnas.1707009114
2. Nishizawa T, Okamoto H, Konishi K, Yoshizawa H, Miyakawa Y, Mayumi M. A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology. Biochem Biophys Res Commun. 1997;241(1):92-97. https://doi.org/10.1006/bbrc.1997.7765
3. Okamoto H. History of discoveries and pathogenicity of TT viruses. Curr Top Microbiol Immunol. 2009;331:1-20. https://doi.org/10.1007/978-3-540-70972-5_1
4. Miyata H, Tsunoda H, Kazi A, Yamada A, Khan MA, Murakami J, Kamahora T, Shiraki K, Hino S. Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus. J Virol. 1999;73(5):3582-3586. https://doi.org/10.1128/JVI.73.5.3582-3586.1999
5. Virus Taxonomy: 2020 Release [Electronic resource]. International Committee on Taxonomy of Viruses [data of access 23 January 2022]. Available from: https://talk.ictvonline.org/taxonomy/
6. Reshetnyak V, Maev I, Burmistrov A, Chekmazov I, Karlovich T. Torque teno virus in liver diseases: On the way towards unity of view. World J Gastroenterol. 2020;26(15):1691-1707. https://doi.org/10.3748/wjg.v26.i15.1691
7. Spandole S, Cimponeriu D, Berca L, Mihăescu Human anelloviruses: an update of molecular, epidemiological and clinical aspects. Arch Virol 2015;160:893-908.
8. Maggi F, Bendinelli M. Immunobiology of the Torque teno viruses and other anelloviruses. Curr Top Microbiol Immunol. 2009;331:65-90. https://doi.org/10.1007/978-3-540-70972-5_5
9. Schmidt L, Jensen B, Walker A, et al. Torque Teno Virus plasma level as novel biomarker of retained immunocompetence in HIV-infected patients. Infection 2021;49:501-509. https://doi.org/10.1007/s15010-020-01573-7
10. Chen T, Väisänen E, Mattila P, Hedman K, Söderlund-Venermo M. Antigenic diversity and seroprevalences of torque teno viruses in children and adults by ORF2based immunoassays. J Gen Virol. 2013;94:409-417. https://doi.org/10.1099/vir.0.046862-0
11. Mankotia D, Irshad M. Development of an Immunoassay for Detection of Torque Teno Virus (TTV) Antibodies Using the N22 Expression Product from TTV Genotype 2. Intervirology. 2017;60(5):207-216. https://doi.org/10.1159/000487481
12. Osipkina OV, Voropaev EV, Mitsura VM, Tereshkov DV, Zyatskov AA, Baranov OYu. Method for molecular diagnostics of TT virus infection: instructions for use. Gomel; 2018. 15 p. (in Russ.).
13. Ninomiya M, Takahashi M, Nishizawa T, Shimosegawa T, Okamoto H. Development of PCR Assays with Nested Primers Specific for Differential Detection of Three Human Anelloviruses and Early Acquisition of Dual or Tri- ple Infection during Infancy. Journal of Clinical Microbiology. 2008;2(46):507-514. https://doi.org/10.1128/JCM.01703-07
14. Hu Y, Al-Moslih M, Al Ali M, Khameneh S, Perkins H, Diaz-Mitoma F, et al. Molecular detection method for all known genotypes of TT virus (TTV) and TTV-like viruses in thalassemia patients and healthy individuals. Journal of clinical microbiology. 2005;43(8):3747-3754. https://doi.org/10.1128/JCM.43.8.3747-3754.2005
Review
For citations:
Osipkina O.V., Voropaev E.V., Mitsura V.M., Tereshkov D.V., Kovalev A.A. Comparison of different DNA detection options for TTV, TTMDV, and TTMV viruses. Health and Ecology Issues. 2022;19(1):102-108. (In Russ.) https://doi.org/10.51523/2708-6011.2022-19-1-13