Analysis of clinico-morphological and molecular-genetic markers in patients with chronic dermatosis followed by face skin atrophy
https://doi.org/10.51523/2708-6011.2020-17-2-21
Abstract
Objective: to identify associations between clinico-morphological and molecular-genetic markers in skin biopsy on the basis of a complex analysis.
Material and methods. Skin punch-biopsies with a diameter from 1 till 4 mm were used as a material for research. To assess the morphological charcateristis of the skin, the histological analysis was carried out; to assess the level of gene expression, real time PCR with reverse transcription was performed.
Results. It has been found that the presence of morphological alterations in the skin of patients with chronic dermatosis is associated with the levels of normalized expression of СOL1A1 gene less than 100 % and/or gene COL1A2 gene less than 200 % and/or gene LOX less than 50 %.
Conclusion. Real time PCR with reverse transcription can be used for objective assessment of the degree of skin alteration in patients with chronic dermatosis.
About the Authors
S. A. Kostiuk
Belarusian Medical Academy for Postgraduate Education
Belarus
Belarus
Svetlana A. Kostiuk – Doctor of Medical Sciences, Professor, Chief researcher at the Science-Research Laboratory of Belarusian Medical Academy for Postgraduate Education
e-mail: s.kostiuk@mail.ru
I. G. Shimanskaya
Belarusian Medical Academy for Postgraduate Education
Belarus
Belarus
Irina G. Shimanskaya – Candidate of Medical Sciences, Associate Professor at the Department of Dermatovenerology and Cosmetology of the SIE «Belarusian Medical Academy of Postgraduate Education»
T. V. Rudenkova
Belarusian Medical Academy for Postgraduate Education
Belarus
Belarus
Tatyana V. Rudenkova – Candidate of Biological Sciences, Leading researcher at the Science-Research Laboratory of Belarusian Medical Academy for Postgraduate Education
O. S. Poluyan
Belarusian Medical Academy for Postgraduate Education
Belarus
Belarus
Olga S. Poluyan – Leading researcher of the Science-Research Laboratory of Belarusian Medical Academy for Postgraduate Education
T. V. Hlinkina
Belarusian Medical Academy for Postgraduate Education
Belarus
Belarus
Tatyana V. Hlinkina – Researcher at the Science-Research Laboratory of Belarusian Medical Academy for Postgraduate Education
References
1. Fett N. Update on morphea: part I. Epidemiology, clinical presentation, and pathogenesis. Journal of the American Academy of Dermatology. 2011;64(2):217-28. doi: 10.1016/j.jaad.2010.05.045
2. Волнухин ВА. Федеральные клинические рекомендации по ведению больных локализованной склеродермией. В: Кубанова АА, ред. Дерматовенерология. Москва, РФ: ДЭКС-Пресс, 2010. с. 52-68.
3. Клименкова НВ, Бич ТА, Шиманская ИГ, Костюк СА. Сравнительная оценка морфологических признаков атрофических изменений соединительной ткани дермы при заболеваниях, сопровождающихся атрофией кожи. Дерматовенерология. Косметология. 2017;3(2):121-34.
4. Меркулов ГА. Курс патологогистологической техники. Москва, РФ: Медицина, 1969. 422 с.
5. Костюк СА, Руденкова ТВ, Шиманская ИГ, Климанкова НВ, Полуян ОС, Глинкина ТВ. Метод определения уровней экспрессии генов, обеспечивающих синтез коллагена и эластина, в биоптатах кожи пациентов с хроническими дерматозами, сопровождающимися атрофией кожи. Дерматовенерология. Косметология. 2017;2:179-87.
Review
For citations:
Kostiuk S.A., Shimanskaya I.G., Rudenkova T.V., Poluyan O.S., Hlinkina T.V. Analysis of clinico-morphological and molecular-genetic markers in patients with chronic dermatosis followed by face skin atrophy. Health and Ecology Issues. 2020;(2):136-142. (In Russ.) https://doi.org/10.51523/2708-6011.2020-17-2-21
Views: 313
Email this article 