Влияние рентгеновского излучения на наномеханические свойства поверхности эритроцитов крыс при гиперхолестериновой диете

Цель исследования. Методом атомно-силовой микроскопии (АСМ) выявить изменения параметров структурных и наномеханических свойств поверхности эритроцитов, вызванные действием рентгеновского излучения in vitro на цельную кровь крыс при гиперхолестериновой диете.

Материалы и методы. Кровь самцов крыс линии Wistar, содержавшихся в течение двух месяцев на гиперхолестериновой диете, подвергали действию рентгеновского излучения (320 кВ) в дозах 1 и 100 Гр. Структурные, упругие и адгезионные свойства поверхности изолированных и фиксированных раствором глутарового альдегида эритроцитов на наномасштабе изучали с помощью атомно-силового микроскопа BioScope Resolve в режиме записи PeakForceQNM на воздухе.

Результаты. Установлено увеличение жесткости поверхности эритроцитов при дозе 1 Гр и ее снижение практически до контрольных значений при дозе 100 Гр, которое сопровождалось увеличением размера средней ячейки мембранного скелета эритроцитов. При этом существенных изменений морфологии, адгезионных свойств и шероховатости рельефа эритроцитов не обнаружено.

Заключение. Полученные данные свидетельствуют о том, что рентгеновское излучение (1–100 Гр) вызывает дозозависимую реорганизацию структуры и изменение жесткости поверхностного слоя эритроцитов на наномасштабе без изменения морфологии клеток у крыс на гиперхолестериновой диете.

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