Experimental myocardial infarction in rats: features of modeling and course within the first 48 hours after ligation of the coronary artery
N. A. Nikulina,
E. A. Dotsenko,
A. M. Nerovnya,
D. P. Salivonchik,
E. N. Platoshkin,
N. V. Nikolaeva,
S. P. Tishkov https://doi.org/10.51523/2708-6011.2020-17-2-13
Abstract
Objective: to identify the features of modeling and give a morphofunctional assessment of the course of experimental myocardial infarction (EMI) in rats within the first 48 hours after ligation of the left coronary artery (LCA). Material and methods. EMI was reproduced according to the method of H. Selye [1] in Jian Ye`s modification [2] by means of LCA ligation in 24 Wistar rats. Electrocardiographical, histological, biochemical studies were performed. The control group consisted of 7 falsely operated animals in which the chest had been opened without manipulation on the LCA. Results. 5-15 minutes after LCA ligation, an ischemic change in the (Q)RST complex was observed as a monophasic curve. The Q wave appeared on the EGG after 1 hour of EMI in 20% cases, after 7 hours in 88% cases, in 100% cases after 27 hours and remained by 48 hours of EMI. Histologically, within the first 7 hours there was acute alterative myocardial damage followed by a quantitative increase in diffusely located necrotic cardiomyocytes (CMC) by 27 hours and the appearance of a clear zone of coagulation necrosis by 48 hours; which was accompanied by corresponding changes in the level of troponin I. Conclusion. The course of EIM after LCA ligation is characterized by the staging of myocardial changes similar to that in humans, which provides a prerequisite for possible extrapolation of the results of the studies to humans.
About the Authors
N. A. Nikulina
Gomel State Medical University
Belarus
Belarus
Natalya A. Nikulina – Candidate of Medical Sciences, Assistant lecturer at the Department of Internal Medicine No.2 with the course of the Faculty of Professional Development and Retraining of the EI «Gomel State Medical University»
e-mail: natallia.nik@mail.ru
E. A. Dotsenko
Belarusian State Medical University
Belarus
Belarus
Edward A. Dotsenko – Doctor of Medical Sciences, Professor, Head of the Department of Propaedeutics of Internal Medicine of the EI «Belarusian State Medical University»
A. M. Nerovnya
Belarusian State Medical University
Belarus
Belarus
Alexander M. Nerovnya – Candidate of Medical Sciences, Associate Professor, Associate Professor at the Department of Pathological Anatomy of the EI «Belarusian State Medical University»
D. P. Salivonchik
Gomel State Medical University
Belarus
Belarus
Dmitry P. Salivonchik – Doctor of Medical Sciences, Associate Professor, Head of the Department of Internal Diseases No.3 with the courses of Radiation Diagnostics, Radiation Therapy, Faculty of Professional Development and Retraining of the EI «Gomel State Medical University»
E. N. Platoshkin
Gomel State Medical University
Belarus
Belarus
Eric N. Platoshkin – Candidate of Medical Sciences, Associate Professor, Head of the Department of Internal Diseases No.2 with the course of the Faculty of Professional Development and Retraining of the EI «Gomel State Medical University»
N. V. Nikolaeva
Gomel State Medical University
Belarus
Belarus
Natalya V. Nikolaeva – Candidate of Medical Sciences, Associate Professor, Associate Professor at Department of Internal Diseases No.2 with the course of the Faculty of Professional Development and Retraining of the EI «Gomel State Medical University»
S. P. Tishkov
Gomel State Medical University
Belarus
Belarus
Sergei P. Tishkov – Assistant lecturer at Department of Internal Diseases No.2 with the course of the Faculty of Professional Development and Retraining of the EI «Gomel State Medical University»
References
1. Selye H, Bajusz E, Grasso S, Mendell P. Sim-ple techniques for the surgical occlusion of coronary vessels in the rat. Angiology. 1960;11:398-407. doi:10.1177/000331976001100505
2. Ye J, Yang L, Sethi R, Copps J, Ramjiawan B, Summers R, Deslauriers R. A new technique of coro-nary artery ligation: experimental myocardial infarction in rats in vivo with reduced mortality. Mol and Cell Biochem. 1997;176(1-2):227-33.
3. Саливончик ДП. Применение гипербариче-ской оксигенации в кардиологической практике. Гомель, РБ; 2010. 196 с.
4. We GC, Siroi MG, Qu R, Liu P, Roulea JL. Ef-fects of quinapril on myocardial function, ventricular remodeling and cardiac cytokine expression in conges-tive heart failure in the rat. Cardiovasc. Drugs and Ther. 2002;16(1):29-36. doi:10.1023/a:1015315531157
5. Михайличенко ВЮ, Пилипчук АА, Самарин СА, Татарчук ПА. Патофизиологические аспекты моделирования инфаркта миокарда у крыс в экс-перименте (данные ангиогенеза и ультразвукового исследования сердца) [Электронных ресурс]. Меж-дународный Журнал Прикладных и Фундамен-тальных Исследований. 2016;11(2):260-263. [дата обращения: 2020 Март 03]. Режим доступа: https://applied-research.ru/ru/article/view?id=10477
6. Захарова ВП, Руденко КВ, Руденко ЕВ, Лев-чишина ЕВ, Третьяк АА. Использование метода MSB в модификации Зербино-Лукасевич для диа-гностики морфофункционального состояния мио-карда. Патология. 2010;7(2):105-106.
7. Гланц, С. Медико-биологическая статистика. Москва, РФ; 1999. 460 с.
Review
For citations:
Nikulina N.A., Dotsenko E.A., Nerovnya A.M., Salivonchik D.P., Platoshkin E.N., Nikolaeva N.V., Tishkov S.P. Experimental myocardial infarction in rats: features of modeling and course within the first 48 hours after ligation of the coronary artery. Health and Ecology Issues. 2020;(2):91-96. (In Russ.) https://doi.org/10.51523/2708-6011.2020-17-2-13
Views: 478
Email this article 