Ascariasis as a factor changing the expression levels of BIRC-5, GLI, VEGF and TP53 suppressor gene in tissue biopsies in rats during the reproduction of experimental C6 glioma

Abstract

Objective: to study ascariasis as a factor leading to changes in the expression levels of BIRC-5, GLI, VEGF and the TP53 suppressor gene in tissue biopsies in rats during the reproduction of experimental C6 glioma.

Material and methods. C6 glioma tumor was modelled in situ in female rats of the first (“control group with tumor”) and second groups (“glioma in combination with ascariasis”). The material was taken on the 14^th, 21^st, 28^th, 35^th days of tumor development in the animals of the first group, on the 7^th (14^th day of tumor development), 14^th (21^st day of tumor development), 21^st (28^th day of tumor development), 28^th day after infection (35^th day of tumor development) in the females of the second group. The animals of the third group were healthy (10 animals). Tissue biopsies were taken from them once.

Results. TThe A. suum invasion of the animals at a dose of 40 eggs per gram of animal body weight increases the expression of BIRC-5, GLI, VEGF genes and the TP53 suppressor gene in the rats with experimental glioma.

Conclusion. Therefore, the authors` experimental model of C6 glioma in situ in rats has showed that the A. suum invasion at a dose of 40 eggs per gram of body weight increases the expression of BIRC-5, GLI, VEGF genes and TP53 suppressor gene in rats with experimental glioma.