Evaluation of the state of the pro-oxidant/antioxidant system in children with hereditary spherocytosis
https://doi.org/10.51523/2708-6011.2021-18-1-8
Abstract
Objective: to assess the state of the pro-oxidant/antioxidant system in children with hereditary spherocytosis (HS) depending on its severity.
Material and methods. The study involved 44 HS patients at the age from 1 to 17 who were divided into 2 groups depending on the disease severity: mild course (n = 24) and moderate or severe course (n = 20). The control group included 23 practically healthy children who were comparable with the main group by gender and age. The state of the pro-oxidant/antioxidant balance of blood plasma was assessed by the method of luminol-dependent chemiluminescence (LDCL) with the determination of the maximum luminescence intensity (Imax, %) and the light sum of chemiluminescence (S, %). The activity of superoxide dismutase (SOD) and catalase was determined in the erythrocytes of the examined children.
Results. On average, the parameters of the pro-oxidant/antioxidant status in the HS patients significantly differed from those of the control group (p <0.05), which corresponded to moderately pronounced oxidative stress. The activity of SOD and catalase in the erythrocytes of the patients was higher as compared with that of the control group (p = 0.0001 and p <0.0001, respectively). The comparison of the severity of oxidative stress depending on HS severity has determined that the degree of stress was more pronounced in patients with moderate or severe course of the disease (p <0.05).
Conclusion. HS patients develop oxidative stress (decreased activity of the antioxidant system associated with increased accumulation of prooxidant substances), the degree of which is higher in patients with a severe course of the disease. This allows of considering plasma LDCL indicators as an additional marker for the assessment of the severity of the disease and of justifying the necessity to include antioxidants in the HS treatment regimen.
About the Authors
E. F. Mitsura
Republican Research Center for Radiation Medicine and Human Ecology
Belarus
Belarus
Ekaterina F. Mitsura, hematologist at the Hematology Department for Children, Gomel
I. A. Novikova
Gomel State Medical University
Belarus
Belarus
Irina A. Novikova, D.Sc. (Medicine), Professor, Head of the Department of Clinical Laboratory Diagnostics, Allergology and Immunology, Gomel
T. S. Petrenko
Gomel State Medical University; Gomel Regional Clinical Hospital
Belarus
Belarus
Tatiana S. Petrenko, Cand. Sc. (Medicine), Associate Professor; laboratory diagnostician, Department of Transfusiology, Gomel
K. S. Makeeva
Gomel State Medical University
Belarus
Belarus
Ksenia S. Makeeva, Senior Lecturer at the Department of Clinical Laboratory Diagnostics, Allergology and Immunology, Gomel
L. I. Volkova
Belarusian Medical Academy for Postgraduate Education
Belarus
Belarus
Lyudmila I. Volkova, Cand. Sc. (Medicine), Associate Professor; Associate Professor at the Department of Child Oncology and Hematology, Minsk
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Review
For citations:
Mitsura E.F., Novikova I.A., Petrenko T.S., Makeeva K.S., Volkova L.I. Evaluation of the state of the pro-oxidant/antioxidant system in children with hereditary spherocytosis. Health and Ecology Issues. 2021;18(1):55-61. (In Russ.) https://doi.org/10.51523/2708-6011.2021-18-1-8
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