THE ACTIVITY OF TRANSMEMBRANE OXYGEN TRANSPORT AND PHYSICAL AND CHEMICAL PROPERTIES OF ERYTHROCYTE MEMBRANES IN PATIENTS WITH DIABETIC FOOT
https://doi.org/10.51523/2708-6011.2016-13-2-14
Abstract
The problem of the pathogenesis of diabetic foot being a complication of diabetes mellitus has been very topical up to now. The supply of oxygen to tissues is one of the key issues of the pathogenesis of diabetic foot. The aim of the work was to study the physical and chemical properties of erythrocyte membranes and activity of oxygen transfer in patients with diabetic foot. It has been found that the activity of oxygen release from erythrocytes is the most significant among the studied parameters in detection of patients with diabetic foot and healthy people. Patients with diabetic foot are divided into two groups in terms of physical and chemical properties of erythrocyte membranes and the activity of oxygen release. The first group of patients with diabetic foot is different from healthy people by the activity of oxygen release and micropolarity, microviscosity of annular lipid pool. The second group of patients with diabetic foot differs from healthy people by micropolarity of annular lipid pool (with 2 concentrations of pyrene), microviscosity of total lipid pool and the activity of oxygen release.
About the Authors
G. A. Stychnevsky
Vitebsk State Order of Peoples` Friendship Medical University
Belarus
Belarus
A. S. Osochuk
Vitebsk State Order of Peoples` Friendship Medical University
Belarus
Belarus
References
1. Houreld, N. N. Shedding light on a new treatment for diabetic wound healing: a review on phototherapy / N. N. Houreld // Scientific World Journal. (electronic journal) - 2014, Article ID 398412, 13 pages Available at: http://dx.doi.org/10.1155/2014/398412 (доступ 24 марта 2016).
2. Diabetic foot disease is associated with reduced erythrocyte deformability / A. Cahn [et al.] //Int. Wound J. (electronic journal) - 2015 May 28. Available at: http://www.ncbi.nlm.nih.gov/pubmed/26018868 (доступ 24 марта 2016).
3. Le Devehat, C. Impaired hemorheological properties in diabetic patients with lower limb arterial ischaemia / C. Le Devehat, T. Khodabandehlou, M. Vimeux // Clin Hemorheol Microcirc. - 2001. - Vol. 25. - № 2. - P. 43-48.
4. Khodabandehlou, T. Hemorheological disturbances as a marker of diabetic foot syndrome deterioration / T. Khodabandehlou, C. Le Dévéhat // Clin Hemorheol Microcirc. - 2004. - Vol. 30. - № 3-4. - P. 219-223.
5. Болдырев, A. A. Введение в биомембранологию: учеб. пособие / А. А. Болдырев. - М., 1990. - 140 с.
6. Осочук, С. С. Окислительная модификация белков и липидов мембран эритроцитов спортсменов циклических видов спорта / С. С.Осочук, А. Ф.Марцинкевич // Вестник ВГМУ. - 2015. - Т. 2, № 2. - С. 47-52.
7. Осочук, С. С. Физико-химические свойства мембран эритроцитов спортсменов циклических видов спорта / С. С. Осочук, А. Ф. Марцинкевич // Вестник ВГМУ. - 2013. - Т. 12, № 3. - С. 25-31.
8. Осочук, С. С. Метод определения интенсивности отдачи кислорода эритроцитами спортсменов циклических видов спорта / С. С. Осочук, А. Ф. Марцинкевич // Лабораторная диагностика Восточная Европа. - 2014. - № 3. - С. 40-46.
9. Dodge, J. The preparation and chemical characteristics of hemoglobin free ghosts of erythrocytes / J. Dodge, C. Mithchell, D. Hanahan // Arch. Biochem. Biophys. - 1963. - Vol. 100. - № 1. - P. 119-130.
10. Protein measurement with the folin phenol reagent / O. H. Lowry [et al.] // The Journal of Biological Chemistry. - 1952. - Vol. 193. - P. 265-275.
11. Добрецов, Г. Е. Флуоресцентные зонды в исследовании клеток, мембран и липопротеинов / Г. Е. Добрецов. - М.: Изд-во «Наука», 1989. - 126 с.
12. Autoinhibition mechanism of the plasma membrane calcium pump isoforms 2 and 4 studied through lipid-protein interaction / I. C. Mangialavori [et al.] // Biochem J. - 2012. - Vol. 443, № 1. - P. 125-131.
Review
For citations:
Stychnevsky G.A., Osochuk A.S. THE ACTIVITY OF TRANSMEMBRANE OXYGEN TRANSPORT AND PHYSICAL AND CHEMICAL PROPERTIES OF ERYTHROCYTE MEMBRANES IN PATIENTS WITH DIABETIC FOOT. Health and Ecology Issues. 2016;(2):68-74. (In Russ.) https://doi.org/10.51523/2708-6011.2016-13-2-14
Views: 302
Email this article 