APPROACHES TO THE ASSESSMENT OF THE BIOLOGICAL AGE BY MEANS OF THE MITOCHONDRIAL DNA ANALYSIS
https://doi.org/10.51523/2708-6011.2015-12-2-13
Abstract
Objective: to reveal genetic markers acting as predictors of various age-associated changes for their early diagnosis. Material and methods. The study included 35 patients diagnosed with chronic gastritis and examined in the Republican Research Center for Radiation Medicine and Human Ecology (Gomel). Polymerase chain reaction (PCR) and electrophoretic DNA fragment analysis were done. Results. We formed a set of primers for the fragment analysis by 2 loci which made it possible to assess the content of mitochondrial DNA in cells as a marker for senescence. Conclusion. We revealed the dependence of the content of mitochondrial DNA (mtDNA) on the calendar age: the increase of the chronological age leads to the decrease of the mtDNA content.
About the Authors
E. V. Voropayev
Gomel State Medical University
Belarus
Belarus
O. Yu. Baranov
Gomel State Medical University
Belarus
Belarus
A. A. Zyatkov
Gomel State Medical University
Belarus
Belarus
O. V. Osipkina
Gomel State Medical University
Belarus
Belarus
References
1. Игамбердиев, А. У. //Сорос. образоват. журн. № 1. - 2000. - С. 32-36.
2. Мазунин, И. О. Митохондрии: жизнь в клетке и ее последствия / И. О. Мазунин, Н. В. Володько //Природа № 10. - 2010. - С. 3-14.
3. Кишкун, А. А. Биологический возраст и старение: возможности определения и пути коррекции / А. А. Кишкун. - М.: ГЭОТАР-Медиа, 2008. - 976 с.
4. Murphy, M. P. How mitochondria produce reactive oxygen species / M. P. Murphy //Biochem. J. - 2009. - Vol. 417 (1). - Р. 1-13.
5. Трубицын, А. Г. Модифицированный вариант митохондриальной теории старения / А. Г. Трубицын //Успехи геронтологии. - 2006. - Вып. 18. - С. 21-28.
Review
For citations:
Voropayev E.V., Baranov O.Yu., Zyatkov A.A., Osipkina O.V. APPROACHES TO THE ASSESSMENT OF THE BIOLOGICAL AGE BY MEANS OF THE MITOCHONDRIAL DNA ANALYSIS. Health and Ecology Issues. 2015;(2):64-68. (In Russ.) https://doi.org/10.51523/2708-6011.2015-12-2-13
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