THE STRUCTURAL AND FUNCTIONAL STATUS OF HEART IN CHRONIC HEART FAILURE IN THE PRELIMINARY USE OF PROBIOTICS

Abstract

Objective: to study the effect of probiotics on the structural and functional state of the heart in stimulated chronic heart failure in rats. Materials and methods. The experiments were performed on male white rats (n = 38). CHF was simulated by double subcutaneous introduction of isoprenaline hydrochloride (Sigma) at a dose of 80 mg/kg at intervals of 24 hours. For histological examination we seized parts of the left ventricular myocardium, prepared cryostat sections of 10 micron thick and stained them by Mallory's method, followed by morphometry using a light microscope MPV-2 («Leitz», Germany) with the digital camera «Leica». The concentration of CRP in serum was measured by agglutination test with latex. Results. It was ascertained that taking the drugs «Dialakt» and «Bifidumbacterin» contribute to preservation of the CMC diameter, which leads to a decrease in fibrosis evidence and areas of undulating deformation and vacuolization of cardiac myocytes (p < 0.05). All the animals with CHF detected certain serum CRP levels (average 172.8 ± 19.2 mg /l), whereas none of the control group revealed it, as it was beyond the sensitivity of the method. In the group of those who had taken «Dialakt», the number of samples with the amount of protein (48 mg/l) reduced to one compared to the animals of group I, while in the group taking «Bifidumbacterin» it reduced to five (86.4 ± 28 mg/l) in the same comparison.