ISOLATION OF HEPATOCYTES
https://doi.org/10.51523/2708-6011.2013-10-4-22
Abstract
Objective: to study the theoretical basis and give the experimental validation of the efficiency of hepatocyte isolation by the method of enzymatic perfusion of the liver. Material and methods. Wistar rats at a mass of 200 g, isolation of hepatocytes by enzymatic perfusion of the liver, light microscopy, assessment of functional cell capacity to synthesize and accumulate glycogen. Results. The article shows the high efficiency of the method of the 2-step adapted enzymatic perfusion of the liver to isolate hepatocytes. The culture of viable hepatocytes with preserved morphological and functional properties was obtained.
About the Authors
A. G. Skuratov
Gomel State Medical University
Belarus
Belarus
D. R. Petreniov
Gomel State Medical University
Belarus
Belarus
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Review
For citations:
Skuratov A.G., Petreniov D.R. ISOLATION OF HEPATOCYTES. Health and Ecology Issues. 2013;(4):114-118. (In Russ.) https://doi.org/10.51523/2708-6011.2013-10-4-22
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