PHENOTYPIC CHANGES OF MESENCHYMAL STEM CELL IN THEIR DIFFERENTIATION IN HEPATOCYTIC DIRECTION
https://doi.org/10.51523/2708-6011.2013-10-2-25
Abstract
Objective: to evaluate the phenotypic changes of mesenchymal stem cells (MSCs) in their differentiation in hepatocytic direction. Materials and methods. Bone-marrow MSCs; hepatocytes isolated by enzymatic perfusion of the liver; directed hepatocytic differentiation of MSCs according to the available and adapted protocols, light microscopy, evaluation of the functional capacity of hepatocyte-like cells to synthesize and accumulate glycogen. Results. The changes observed in the phenotype during differentiation stages indicate the presence of cells differentiated in hepatocytic direction in the culture. MSCs first gained a spindle-shaped phenotype, which then changed to a polygonal membrane with numerous appendages. The clusters of hepatocyte-like cells positive for glycogen were found. The presence of growth factors, differentiation media and allocated primary hepatocytes had a «stabilizing» effect on the culture of MSCs.
About the Authors
A. G. Skuratov
Gomel State Medical University
Belarus
Belarus
D. R. Petrenyov
Gomel State Medical University
Belarus
Belarus
A. N. Kondrachuk
Gomel State Medical University
Belarus
Belarus
Y. V. Voropayev
Gomel State Medical University
Belarus
Belarus
References
1. Spheroid formation and differentiation into hepatocyte-like cells of rat mesenchymalstem cell induced by co-culture with liver cells / Q. Zhang [et al.] // DNA CellBiol. - 2007. - № 26. - P. 497-503.
2. Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells / C. Lange [et al.] // World J Gastroenterol. - 2006. - № 12. - P. 2394-2397.
3. In vitro differentiation of mouse bone marrow stromal stem cells into hepatocytes induced by conditioned culture medium of hepatocytes / Y. Chen [et al.] // J. Cell. Biochem. - 2007. - № 102. - P. 52-63.
4. Hepatic stellate cells modulate differentiation of bone marrow mesenchymal cells into hepatocyte-like cells / X. Deng [et. al.] // J. Cell. Physiol. - 2008. - № 217. - P. 138-144.
5. Clonal mesenchymal stem cells derived from human bone marrow can differentiate into hepatocyte-like cells in injured livers of SCID mice / X. R. Tao [et al.] // J. Cell. Biochem. - 2009. - № 108. - P. 693-704.
6. A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone / H. Zhu [et al.] // Nat. Protoc. - 2010. - № 5(3). - P. 550-560.
7. Seglen, P. O. Preparation of rat liver cells / P. O. Seglen // Methods Cell Biol. - 1976. - № 13. - Р. 29-83.
8. Дифференцировка мезенхимальных стволовых клеток костного мозга в гепатоцитподобные клетки in vitro / Я. И. Исайкина [и др.] // Вести Национальной Академии Наук Беларуси. - 2011. - № 1. - С. 10-15.
9. Liver-specific gene expression in mesenchymal stem cells is induced by liver cells / C. Lange [et al.] // World J Gastroenterol. - 2005. - № 11(29). - P. 4497-4504.
10. Hepatic Differentiation of Mesenchymal Stem Cells: In Vitro Strategies / S. Snykers [et al.] // in Mesenchymal Stem Cell Assays and Applications, Methods in Molecular Biology. - Vol. 698 - P. 305-314.
Review
For citations:
Skuratov A.G., Petrenyov D.R., Kondrachuk A.N., Voropayev Y.V. PHENOTYPIC CHANGES OF MESENCHYMAL STEM CELL IN THEIR DIFFERENTIATION IN HEPATOCYTIC DIRECTION. Health and Ecology Issues. 2013;(2):134-138. (In Russ.) https://doi.org/10.51523/2708-6011.2013-10-2-25
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