STUDY OF EXOGENOUS MESENCHYMAL STEM CELL MIGRATION AT EXPERIMENT

Objective: to study the migration of mesenchymal stem cells introduced into the body of laboratory animals with induced toxic hepatitis. Materials and Methods: Wistar laboratory rats; CCl₄-induced toxic hepatitis, mesenchymal stem cells from adipose tissue (isolation, cultivation and coloring by fluorescence PKH 67), the introduction of mesenchymal stem cells in rats, fluorescent microscopy of the organ cryo- images. Results: The analysis of the rats` fluorescence microscopy images revealed the presence of bright yellow-green cell size fluorescence foci (presumably of marked mesenchymal stem cells) caused by greenish cytoplasm autofluorescence and red nucleus fluorescence. It was found that the prevalence of these foci in various organs was differed. The highest density of the foci was observed in spleen and liver in chronic hepatitis. In kidneys, myocardium, lungs, in rare cases, there were isolated foci of bright fluorescence and cases of doubtfully positive cells. Conclusion: The intravenous administration of marked mesenchymal stem cells revealed specific fluorescent foci in the spleen of both the healthy and sick animals and in the liver of the rats with chronic hepatitis. This may be indicative of MSC`s tropism to spleen and injured liver. There was a trend to increase the concentration of the foci from the first to the fifth day of observation.

крысы линии Вистар, CCl₄-модель хронического гепатита, мезенхимальные стволовые клетки, флуоресцентная микроскопия, Wistar rats, CCl₄-model of chronic hepatitis, mesenchymal stem cells, fluorescent microscopy

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