COMPARATIVE ANALYSIS OF METHYLATION STATUS OF SUPPRESSOR GENES IN THE PROSTATE GLAND TISSUE IN PATIENTS WITH BENIGN PATHOLOGY, PROSTATE CANCER AND IN PATIENTS WITHOUT PATHOLOGY

The comparative analysis of the methylation status of promoter regions of eleven different suppressor genes has been carried out in the groups of patients with benign prostatic hyperplasia, prostate cancer and in patients without pathology with the use of methylation-specific polymerase chain reaction. The group without pathology revealed no methylation. The group of the patients with benign prostatic hyperplasia in most cases revealed either no methylation or it occurred in only one of the eleven genes. Methylation of two or more genes was found in five cases which in further histological examination showed morphological evidence of in situ carcinoma . As a result of the comparative analysis of the study groups five candidate genes were selected for the genetic markers panel for differential prostate pathology diagnosis.

доброкачественная гиперплазия предстательной железы, рак предстательной железы, гиперметилирование генов-супрессоров, полимеразная цепная реакция, benign prostatic hyperplasia, prostate cancer, hypermethylation of suppressor genes, polymerase chain reaction

1. Alterations in DNA methylation: a fundamental aspect of neoplasia / I. V. Baylin [et al.] // Adv. Cancer Res. - 1998. - Vol. 72. - P. 141-196.

2. Herman, J. G. Hypermethylation of tumor suppressor genes in cancer / J. G. Herman // Sem. Cancer Biol. - 1999. - Vol. 9. - P. 359-367.

3. Метилирование ДНК и канцерогенез / Б. А. Лихтенштейн [и др.] // Биохимия. - 2001. - № 66. - C. 235-255.

4. Detection of Aberrant p16 Methylation in the Plasma and Serum of Liver Cancer Patients / D. E. Wong [et al.] // Cancer Research. - 1999. - Vol. 59. - P. 71-73.

5. Detection of Aberrant Promoter Hypermethylation of Tumor Suppressor Genes in Serum DNA from Non-Small Cell Lung Cancer Patients / J. K. Esteller [et al.] // Cancer Research. - 1999. - Vol. 59. - P. 67-70.

6. Detection of Bladder Cancer in Urine by a Tumor Suppressor Gene Hypermethylation Panel / M. R. Dulaimi [et al.] // Clinical Cancer Research. - 2004. - Vol. 10. - P. 1887-1893.

7. Xing, M. Minireview: Gene Methylation in Thyroid Tumorigenesis / M. Xing // Endocrinology. - 2007. - Vol. 148, № 3. - P. 948-953.

8. Epigenetics in Prostate Cancer: Biologic and Clinical Relevance / F. S. Jeroґnimo [et al.] // European Urology. - 2011. - Vol. 60. - P. 753-766.

9. Molecular Biomarker in Prostate Cancer: The Role of CpGIsland Hypermethylation / F. A. Bastian [et al.] // European Urology. - 2004. - Vol. 46. - P. 698-708.