Etiological verification of infectious exacerbation of chronic obstructive lung disease using molecular genetics methods

Abstract

Objective: To study the possibilities of the method of determining the 16S rRNA gene of bacteria in the determination of the bacterial spectrum of acute exacerbation of chronic obstructive pulmonary disease (COPD) using a protected material sampling Materials and Methods: 68 Hospitalized patients with acute exacerbation of COPD were prospectively evaluated. Molecular genetic determination of the 16S rRNA gene of bacteria and cultural microbiological techniques from sputum and a bronchial tree material obtained using a protected brush biopsy were used. Results: Bacterial agents were detected in 91,2 % of cases of acute exacerbation of COPD. Pseudomonas aeruginosa was detected in the biopsy material 1.8 times more often than with the use of routine methods. Patterns of COPD with frequent exacerbations, infectious exacerbations and Pseudomonas etiology of exacerbations were determined. Conclusion: Using the method of molecular-genetic determination of the 16S rRNA gene allows to verify the bacterial spectrum of acute exacerbation of COPD.