MOLECULAR-GENETIC DIAGNOSTICS OF HUMAN PAPILLOMAVIRUS UNDER DYSPLASIA AND CERVICAL CARCINOMA

Abstract

80 patients with cervical carcinoma and 89 patients with cervical intraepithelial neoplasia (CIN) were examined for the presence of HPV serotypes with the assessment of the level of viral load. The method of polymerase chain reaction with hybridization-fluorescence detection in the regimen of «real time» was used. It was established, that 94,7 % of the patients with cervical carcinoma were HPV-positive, in the prevalent number of cases (48,7 %) one serotype was revealed. The patients displayed HPV infection in 80,9 % of cases with CIN II-III, in 66,7 % in the presence of two genotypes. The increased contents of human papillomavirus were diagnosed in 86,4 % of the patients with cervical carcinoma and in 70,8 % of the patients with CIN II-III. The quantity of the revealed HPV serotypes, presence of genital infections and moderate differential tumor (R = 0,706473, p < 0,005) were found interrelated, there is also a correlation between the prevalence of the tumor process and the quantity of the diagnosed HPV genotypes (R = 0,517676, p < 0,05).