EFFECT OF GLUCOSE CONCENTRATION ON THE LEVEL OF PROBE FLUORESCENTION OF BULL AND SERUM ALBUMINS

Abstract

The rise of glucose concentration in protein solutions gives a decrease in intensity indices of probe fluorescention. This change of probe fluorescention is most probably caused by the decreased quantity of ANS binded with the protein. Thus, one can suppose that the rise of glucose concentration in blood initiates the decrease in albumin binding capacity towards other ligands. The rise of glucose concentration makes the intensity indices of protein fluorescention decrease. The ability of glucose to bind with protein initiates the rearrangement in globule electron density, which promotes intramolecular conformational changes in protein and the decrease of the intensity indices of tryptophan fluorescention to testify that.