zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2009-6-2-5zdor-815Research ArticleКЛИНИЧЕСКАЯ МЕДИЦИНАCLINICAL MEDICINEМОРФОЛОГИЧЕСКАЯ И ИММУНОГИСТОХИМИЧЕСКАЯ ХАРАКТЕРИСТИКА ИНТЕРСТИЦИАЛЬНОЙ ТКАНИ ПОЧЕК ПРИ ПЕРВИЧНЫХ ГЛОМЕРУЛОПАТИЯХMORPHOLOGICAL AND IMMUNOHISTOCHEMICAL DESCRIPTION OF INTERSTITIAL TISSUE IN PRIMARY GLOMERULOPATHYСавошВ. В.SavoshV. V.noemail@neicon.ruЛетковскаяТ. А.LiatkouskayaT. A.noemail@neicon.ruЧерствыйЕ. Д.CherstvoyE. D.noemail@neicon.ruБелорусский государственный медицинский университетBelurussian State Medical University200928062009022630Copyright © Савош В.В., Летковская Т.А., Черствый Е.Д., 20092009Савош В.В., Летковская Т.А., Черствый Е.Д.Savosh V.V., Liatkouskaya T.A., Cherstvoy E.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/815

Работа посвящена изучению морфологических изменений интерстиция и канальцев почки, а также экспрессии гладкомышечного актина-а, трансформирующего фактора роста-β и некоторых компонентов экстрацеллюлярного матрикса (коллагена IV типа, фибронектина) при первичных гломерулопатиях у детей. В результате комплексного анализа морфологических и клинических данных была выявлена статистически достоверная связь между уровнями экспрессии a-SMA и ТФР-β и количеством в почечном интерстиции коллагена IV типа и фибронектина. Также установлена взаимосвязь выраженности экспрессии a-SMA и ТФР-β от уровня суточной экскреции белка, что позволяет отнести протеинурию к неблагоприятным фактора в прогрессировании хронических болезней почек.

The purpose of our study was to ascertain the possible relationships between number of interstitial myofibroblasts, TGF-b and interstitial deposition of matrix proteins in the renal tissue of patients with primary glomerulopathies with the use of immunostaining. There was a significant correlation between level of proteinuria and interstitial a-SMA expression, and tubular expression of TGF-b. Interstitial a-SMA expression was significantly higher in patients with nephrotic proteinuria level compared with patients with urinary protein excretion less then 1 g/1. A highly significant correlation was also evident between the number of interstitial myofibroblasts and interstitial deposition of collagen types IV and fibronectin (p < 0,05). We found that tubulointerstitial changes in the renal tissue of patients with proteinuric glomerulopathies were accompanied by increases number of interstitial myofibroflasts and expression of collagen type IV and fibronectin.

гломерулопатиятубулоинтерстициальные измененияфиброзмиофибробластыэкстрацеллюлярный матриксglomerulopathytubulointerstitial changesfibrosismyofibroblastsextracellular matrixReferencesAllison, A. Eddy Molecular Insights Into Renal Interstitial Fibrosis / A. Allison // J. Am. Soc. Nephrol. - 1996. - Vol. 7. - P. 2495-2508.Allison, A. Eddy Molecular Insights Into Renal Interstitial Fibrosis / A. Allison // J. Am. Soc. Nephrol. - 1996. - Vol. 7. - P. 2495-2508.Agnes, B. Fogo Mechanisms of progression of chronic kidney disease / B. Agnes // Pediatr Nephrol. - 2007. - Vol. 22. - P. 2011-2022.Agnes, B. Fogo Mechanisms of progression of chronic kidney disease / B. Agnes // Pediatr Nephrol. - 2007. - Vol. 22. - P. 2011-2022.Risdon, R. A. Relationship between renal function and histologic changes found in renal biopsy specimens from patients with persistent glomerulonephritis / R. A. Risdon, J. C. Sloper, H. E. de Vardener // Lancet. - 1968. - Vol. 2. - Р. 363-366.Risdon, R. A. Relationship between renal function and histologic changes found in renal biopsy specimens from patients with persistent glomerulonephritis / R. A. Risdon, J. C. Sloper, H. E. de Vardener // Lancet. - 1968. - Vol. 2. - Р. 363-366.Nath, K. A. The tubulointerstitium in progressive renal disease / K. A. Nath // Kidney International. - 1998. - Vol. 54. - P. 992-994.Nath, K. A. The tubulointerstitium in progressive renal disease / K. A. Nath // Kidney International. - 1998. - Vol. 54. - P. 992-994.Masaomi, N. Mechanisms of Tubulointerstitial Injury in the Kidney: Final Common Pathways to End-stage Renal Failure / N. Masaomi // Internal Medicine. - 2004. - Vol. 43. - P. 9-17.Masaomi, N. Mechanisms of Tubulointerstitial Injury in the Kidney: Final Common Pathways to End-stage Renal Failure / N. Masaomi // Internal Medicine. - 2004. - Vol. 43. - P. 9-17.D’Amico, G. Tubulointerstitial damage in glomerular diseases: its role in the progression of renal damage / G. D’Amico, F. Ferrario, M. P. Rastaldi // Am J Kidney Dis. - 1995. - Vol. 26. - P. 124-132.D’Amico, G. Tubulointerstitial damage in glomerular diseases: its role in the progression of renal damage / G. D’Amico, F. Ferrario, M. P. Rastaldi // Am J Kidney Dis. - 1995. - Vol. 26. - P. 124-132.Renal Fibrosis: Extracellular Matrix Microenvironment Regulates Migratory Behavior of Activated Tubular Epithelial Cells // M. Zeisberg [et al.] // Am J Pathol. - 2002. - Vol. 160. - P. 2001-2008.Renal Fibrosis: Extracellular Matrix Microenvironment Regulates Migratory Behavior of Activated Tubular Epithelial Cells // M. Zeisberg [et al.] // Am J Pathol. - 2002. - Vol. 160. - P. 2001-2008.Muchaneta-Kubara, E. C. El Myofibroblast phenotypes expression in experimental renal scarring / E. C. Muchaneta-Kubara, A. M. Nahas // Nephrol Dial Transplant. - 1997. - Vol. 12. - P. 904-915.Muchaneta-Kubara, E. C. El Myofibroblast phenotypes expression in experimental renal scarring / E. C. Muchaneta-Kubara, A. M. Nahas // Nephrol Dial Transplant. - 1997. - Vol. 12. - P. 904-915.Furness, P. N. Extracellular matrix and the kidney / P. N. Furness // J Clin Pathol. - 1996. - Vol. 49. - P. 355-359.Furness, P. N. Extracellular matrix and the kidney / P. N. Furness // J Clin Pathol. - 1996. - Vol. 49. - P. 355-359.Epithelial-mesenchymal transition (EMT) of renal tubular cells in canine glomerulonephritis / L. Aresu [et al.] // Virchows Arch. - 2007. - Vol. 451. - P. 937-942.Epithelial-mesenchymal transition (EMT) of renal tubular cells in canine glomerulonephritis / L. Aresu [et al.] // Virchows Arch. - 2007. - Vol. 451. - P. 937-942.Normal and Pathologic Soft Tissue Remodeling: Role of the Myofibroblast, with Special Emphasis on Liver and Kidney Fibrosis // A. Desmoulière [et al.] // Laboratory nvestigation. - 2003. - Vol. 83, № 1. - P. 1689-1707.Normal and Pathologic Soft Tissue Remodeling: Role of the Myofibroblast, with Special Emphasis on Liver and Kidney Fibrosis // A. Desmoulière [et al.] // Laboratory nvestigation. - 2003. - Vol. 83, № 1. - P. 1689-1707.Фибронектин мочи как показатель процессов фиброзирования в почках при нефрите / Л. В. Козловская [и др.] // Терапевтический архив. - 1999. - № 6. - С. 34-38.Фибронектин мочи как показатель процессов фиброзирования в почках при нефрите / Л. В. Козловская [и др.] // Терапевтический архив. - 1999. - № 6. - С. 34-38.Transforming growth factor-b1 differentially mediates fibronectin and inflammatory cytokine expression in kidney tubular cells // W. Qi [et al.] // Am J Physiol Renal Physiol. - 2006. - Vol. 291. - P. 1070-1077.Transforming growth factor-b1 differentially mediates fibronectin and inflammatory cytokine expression in kidney tubular cells // W. Qi [et al.] // Am J Physiol Renal Physiol. - 2006. - Vol. 291. - P. 1070-1077.Relevance of Periglomerular Myofibroblasts in Progression of Human Glomerulonephritis / S. Oba [et al.] // Am J Kidney Dis. - 1998. - Vol. 32, № 3. - P. 419-425.Relevance of Periglomerular Myofibroblasts in Progression of Human Glomerulonephritis / S. Oba [et al.] // Am J Kidney Dis. - 1998. - Vol. 32, № 3. - P. 419-425.Effects of Transforming Growth Factor-b1 on Renal Extracellular Matrix Components and Their Regulating Proteins // J. A. Douthwait [et al.] // J Am Soc Nephrol. - 1999. - Vol. 10. - P. 2109-2119.Effects of Transforming Growth Factor-b1 on Renal Extracellular Matrix Components and Their Regulating Proteins // J. A. Douthwait [et al.] // J Am Soc Nephrol. - 1999. - Vol. 10. - P. 2109-2119.

The authors declare that there are no conflicts of interest present.