zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2008-5-1-31zdor-678Research ArticleНОВЫЕ ТЕХНОЛОГИИ В МЕДИЦИНЕАСМ ЭЛАСТОГРАФИЯ - НОВЫЙ МЕТОД БИОМЕДИЦИНСКИХ ИССЛЕДОВАНИЙAFM ELASTOGRAPHY IN A NEW BIO-MEDICAL INVESTIGATIOКузнецоваТ. Г.KuznetsovaT. G.noemail@neicon.ruСтародубцеваМ. Н.StarodubtsevaM. N.noemail@neicon.ruГомельский государственный медицинский университетGomel State Medical University20082803200801143149Copyright © Кузнецова Т.Г., Стародубцева М.Н., 20082008Кузнецова Т.Г., Стародубцева М.Н.Kuznetsova T.G., Starodubtseva M.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/678

В статье представлен обзор современных исследований в области изучения наномеханических свойств клеток методами атомно-силовой микроскопии. Определены перспективы развития клеточной эластографии и круг медико-биологических проблем, решаемых с ее использованием.

The paper reviews recent atomic force microscopy findings in the area of cell nanomechanics and determines the perspectives of elastography as a new bio-medical technology.

атомно-силовая микроскопия (АСМ)механические свойства клетокatomic force microscopy (AFM)mechanical properties of the cellsReferencesКузнецова, Т. Г. Методологические проблемы изучения наномеханических свойств живых клеток / Т. Г.Кузнецова, М. Н. Стародубцева, Н. И. Егоренков // Проблемы здоровья и экологии. - 2007. - № 3 (13). - С. 103-111.Кузнецова, Т. Г. Методологические проблемы изучения наномеханических свойств живых клеток / Т. Г.Кузнецова, М. Н. Стародубцева, Н. И. Егоренков // Проблемы здоровья и экологии. - 2007. - № 3 (13). - С. 103-111.Barbee, K. A. Shear stress-induced reorganization of the surface topography of living endothelial cells imaged by atomic force microscopy / K. A. Barbee, P. F.Davies, R. Lal // Circulation Research. - 1994.- Vol. 74. - P. 163-171.Barbee, K. A. Shear stress-induced reorganization of the surface topography of living endothelial cells imaged by atomic force microscopy / K. A. Barbee, P. F.Davies, R. Lal // Circulation Research. - 1994.- Vol. 74. - P. 163-171.Barbee, K. A. Changes in surface topography in endothelial monolayers with time at confluence: influence on subcellular shear stress distribution due to flow / K. A. Barbee // Biochemistry and Cell Biology. - 1995. - Vol. 73. - P. 501-505.Barbee, K. A. Changes in surface topography in endothelial monolayers with time at confluence: influence on subcellular shear stress distribution due to flow / K. A. Barbee // Biochemistry and Cell Biology. - 1995. - Vol. 73. - P. 501-505.Kinetic study on the elastic change of vascular endothelial cells on collagen matrices by atomic force microscopy/ H. Sato [et al.] // Colloids and Surfaces B: Biointerfaces. - 2004. - Vol. 34(2). - P. 141-146.Kinetic study on the elastic change of vascular endothelial cells on collagen matrices by atomic force microscopy/ H. Sato [et al.] // Colloids and Surfaces B: Biointerfaces. - 2004. - Vol. 34(2). - P. 141-146.Experimental and numerical analyses of local mechanical properties measured by atomic force microscopy for sheared endothelial cells / T. Ohashi [et al.] // Bio-Medical Materials and Engineering. - 2002. - Vol. 12. - P. 319-327.Experimental and numerical analyses of local mechanical properties measured by atomic force microscopy for sheared endothelial cells / T. Ohashi [et al.] // Bio-Medical Materials and Engineering. - 2002. - Vol. 12. - P. 319-327.Pesen, D. Micromechanical Architecture of the Endothelial Cell Cortex / D. Pesen, J. H.Hoh // Biophysical Journal. - 2005. - Vol. 88. - P. 670-679.Pesen, D. Micromechanical Architecture of the Endothelial Cell Cortex / D. Pesen, J. H.Hoh // Biophysical Journal. - 2005. - Vol. 88. - P. 670-679.Miyazaki, H. Atomic force microscopic measurement of the mechanical properties of intact endothelial cells in fresh arteries / H. Miyazaki, K. Hayashi // Medical and Biological Engineering and Computing. - 1999. - Vol. 37(4). - P. 530-536.Miyazaki, H. Atomic force microscopic measurement of the mechanical properties of intact endothelial cells in fresh arteries / H. Miyazaki, K. Hayashi // Medical and Biological Engineering and Computing. - 1999. - Vol. 37(4). - P. 530-536.Fritz, M. Granula motion and membrane spreading during activation of human platelets imaged by atomic force microscopy / M. Fritz, M. Radmacher, H. E. Gaub // Biophysical Journal. - 1994. - Vol. 66(5). - P. 1328-1334.Fritz, M. Granula motion and membrane spreading during activation of human platelets imaged by atomic force microscopy / M. Fritz, M. Radmacher, H. E. Gaub // Biophysical Journal. - 1994. - Vol. 66(5). - P. 1328-1334.Fritz, M. In vitro activation of human plateles triggered and probed by atomic force microscopy / M. Fritz, M. Radmacher, H. E. Gaub // Experimental Cell Research. - 1995. - Vol. 205. - P. 187-190.Fritz, M. In vitro activation of human plateles triggered and probed by atomic force microscopy / M. Fritz, M. Radmacher, H. E. Gaub // Experimental Cell Research. - 1995. - Vol. 205. - P. 187-190.Measuring the viscoelastic properties of human platelets with the atomic force microscope / M.Radmacher [et al.] // Biophysical Journal. - 1996. - Vol. 70(1). - P. 556-567.Measuring the viscoelastic properties of human platelets with the atomic force microscope / M.Radmacher [et al.] // Biophysical Journal. - 1996. - Vol. 70(1). - P. 556-567.Elastic properties of living fibroblasts as imaged using force modulation mode in atomic force microscopy / S. Sasaki [et al.] // Archives of Histology and Cytology. - 1998. - Vol. 61(1). - P. 57-63.Elastic properties of living fibroblasts as imaged using force modulation mode in atomic force microscopy / S. Sasaki [et al.] // Archives of Histology and Cytology. - 1998. - Vol. 61(1). - P. 57-63.Nagayama, M. Drastic change of local stiffness distribution correlating to cell migration in living fibroblasts / M. Nagayama, H. Haga, K. Kawabata // Cell Motility and the Cytoskeleton. - 2001. - Vol. 50(4). - P. 173-179.Nagayama, M. Drastic change of local stiffness distribution correlating to cell migration in living fibroblasts / M. Nagayama, H. Haga, K. Kawabata // Cell Motility and the Cytoskeleton. - 2001. - Vol. 50(4). - P. 173-179.Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM / H. Haga [et al.] // Journal of Electron Microscopy (Tokyo). - 2000. - Vol. 49(3). - P. 473-481.Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM / H. Haga [et al.] // Journal of Electron Microscopy (Tokyo). - 2000. - Vol. 49(3). - P. 473-481.Rotsch, C. Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by using atomic force microscopy / C. Rotsch, K. Jacobson, R. Radmacher// Proceedings of the National Academy Sciences USA. - 1999. - Vol. 96. - P. 921-926.Rotsch, C. Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by using atomic force microscopy / C. Rotsch, K. Jacobson, R. Radmacher// Proceedings of the National Academy Sciences USA. - 1999. - Vol. 96. - P. 921-926.Quantitative analysis of the viscoelastic properties of thin regions of fibroblasts using atomic force microscopy / R. E. Mahaffy [et al.] // Biophysical Journal. - 2004. - Vol. 86. - P. 1777-1793.Quantitative analysis of the viscoelastic properties of thin regions of fibroblasts using atomic force microscopy / R. E. Mahaffy [et al.] // Biophysical Journal. - 2004. - Vol. 86. - P. 1777-1793.Gradient of Rigidity in the Lamellipodia of Migrating Cells Revealed by Atomic Force Microscopy / V. M. Laurent [et al] // Biophysical Journal. - 2005. - Vol. 89. - P. 667-675.Gradient of Rigidity in the Lamellipodia of Migrating Cells Revealed by Atomic Force Microscopy / V. M. Laurent [et al] // Biophysical Journal. - 2005. - Vol. 89. - P. 667-675.Osteoblast elastic modulus measured by atomic force microscopy is substrate dependent / E. Takai [et al] // Annals of Biomedical Engineering. - 2005. - Vol. 33(7). - P. 963-971.Osteoblast elastic modulus measured by atomic force microscopy is substrate dependent / E. Takai [et al] // Annals of Biomedical Engineering. - 2005. - Vol. 33(7). - P. 963-971.Characterization of dynamic cellular adhesion of osteoblasts using atomic force microscopy / A. Simon [et al.] // Cytometry. - 2003. - Vol. A 54(1). - P. 36-47.Characterization of dynamic cellular adhesion of osteoblasts using atomic force microscopy / A. Simon [et al.] // Cytometry. - 2003. - Vol. A 54(1). - P. 36-47.Substrate dependent differences in morphology and elasticity of living osteoblasts investigated by atomic force microscopy / J. Domke [et al.] // Colloids and Surfaces B: Biointerfaces. - 2000. - Vol. 19(4). - P. 367-379.Substrate dependent differences in morphology and elasticity of living osteoblasts investigated by atomic force microscopy / J. Domke [et al.] // Colloids and Surfaces B: Biointerfaces. - 2000. - Vol. 19(4). - P. 367-379.Local mechanical properties of mouse outer hair cells: atomic force microscopic study / M. Murakoshi [et al.] // Auris, Nasus, Larynx. - 2006. - Vol. 33(2). - P.149-157.Local mechanical properties of mouse outer hair cells: atomic force microscopic study / M. Murakoshi [et al.] // Auris, Nasus, Larynx. - 2006. - Vol. 33(2). - P.149-157.Tolomeo, J. F. Mechanical properties of the lateral cortex of mammalian auditory outer hair cells / J. F. Tolomeo, C. R. Steele, M. C. Holley // Biophysical Journal. - 1996. - Vol. 71(1). - P. 421-429.Tolomeo, J. F. Mechanical properties of the lateral cortex of mammalian auditory outer hair cells / J. F. Tolomeo, C. R. Steele, M. C. Holley // Biophysical Journal. - 1996. - Vol. 71(1). - P. 421-429.Sugawara, M. Local mechanical properties of guinea pig outer hair cells measured by atomic force microscopy / M. Sugawara, Y. Ishida, H. Wada // Hearing Research. - 2002. - Vol. 174(1-2). - P. 222-229.Sugawara, M. Local mechanical properties of guinea pig outer hair cells measured by atomic force microscopy / M. Sugawara, Y. Ishida, H. Wada // Hearing Research. - 2002. - Vol. 174(1-2). - P. 222-229.Relationship between the local stiffness of the outer hair cell along the cell axis and its ultrastructure observed by atomic force microscopy / H. Wada [et al.] // Hearing Research. - 2003. - Vol. 177 (1-2). - P. 61-70.Relationship between the local stiffness of the outer hair cell along the cell axis and its ultrastructure observed by atomic force microscopy / H. Wada [et al.] // Hearing Research. - 2003. - Vol. 177 (1-2). - P. 61-70.Sugawara, M. Mechanical properties of sensory and supporting cells in the organ of Corti of the guinea pig cochlea-study by atomic force microscopy / M. Sugawara, Y. Ishida, H. Wada // Hearing Research. - 2004. - Vol. 192(1-2). - P. 57-64.Sugawara, M. Mechanical properties of sensory and supporting cells in the organ of Corti of the guinea pig cochlea-study by atomic force microscopy / M. Sugawara, Y. Ishida, H. Wada // Hearing Research. - 2004. - Vol. 192(1-2). - P. 57-64.Shroff, S. G. Dynamic micromechanical properties of cultured rat atrial myocytes measured by atomic force microscopy / S. G. Shroff, D. R. Saner, R. Lal // American Journal of Physiology. - 1995. - Vol. 269 (Cell Physiology 38). - P. 286-292.Shroff, S. G. Dynamic micromechanical properties of cultured rat atrial myocytes measured by atomic force microscopy / S. G. Shroff, D. R. Saner, R. Lal // American Journal of Physiology. - 1995. - Vol. 269 (Cell Physiology 38). - P. 286-292.AFM imaging and elasticity measurements on living rat liver macrophages / C. Rotsch, [et al.] // Cell Biology International. - 1997. - Vol. 21(11). - P. 685-696.AFM imaging and elasticity measurements on living rat liver macrophages / C. Rotsch, [et al.] // Cell Biology International. - 1997. - Vol. 21(11). - P. 685-696.Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope / U.G. Hofmann [et al.] // Journal of Structural Biology. - 1997. - Vol. 119. - P. 84-91.Investigating the cytoskeleton of chicken cardiocytes with the atomic force microscope / U.G. Hofmann [et al.] // Journal of Structural Biology. - 1997. - Vol. 119. - P. 84-91.Wu, H. W. Mechanical properties of L929 fibroblasts measured by atomic force microscopy: effects of anticytoskeletal drugs and membrane crosslinking / H. W. Wu, T. Kuhn, V. N. Moy // Scanning. - 1998. - Vol. 20(5). - P. 389-397.Wu, H. W. Mechanical properties of L929 fibroblasts measured by atomic force microscopy: effects of anticytoskeletal drugs and membrane crosslinking / H. W. Wu, T. Kuhn, V. N. Moy // Scanning. - 1998. - Vol. 20(5). - P. 389-397.Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation / A. M. Collinsworth [et al.] // American Journal of Physiology. - Cell Physiology. - 2002. - Vol. 283. - P. 1219-1227.Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation / A. M. Collinsworth [et al.] // American Journal of Physiology. - Cell Physiology. - 2002. - Vol. 283. - P. 1219-1227.A comparative atomic force microscopy study on living skin fibroblasts and liver endothelial cells / F. Braet [et al.] // Journal of Electron Microscopy (Tokyo). - 2001. - Vol. 50(4). - P. 283-290.A comparative atomic force microscopy study on living skin fibroblasts and liver endothelial cells / F. Braet [et al.] // Journal of Electron Microscopy (Tokyo). - 2001. - Vol. 50(4). - P. 283-290.Rotsch, C. Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study / C. Rotsch, R. Radmacher // Biophysical Journal. - 2000. - Vol. 78(1). - P. 520-535.Rotsch, C. Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study / C. Rotsch, R. Radmacher // Biophysical Journal. - 2000. - Vol. 78(1). - P. 520-535.Zhang, S. Stretch-induced nitric oxide modulates mechanical properties of skeletal muscle cells / S. Zhang, W. E. Kraus, G. A. Truskey // American Journal of Physiology. - Cell Physiology. - 2004. - Vol. 287. - P. 292-299.Zhang, S. Stretch-induced nitric oxide modulates mechanical properties of skeletal muscle cells / S. Zhang, W. E. Kraus, G. A. Truskey // American Journal of Physiology. - Cell Physiology. - 2004. - Vol. 287. - P. 292-299.Aging increases stiffness of cardiac myocytes measured by atomic force microscopy nanoindentation / S.C. Lieber [et al] // American Journal of Physiology - Heart and Circulatory Physiology. - 2004. - Vol. 287. - P. 645-651.Aging increases stiffness of cardiac myocytes measured by atomic force microscopy nanoindentation / S.C. Lieber [et al] // American Journal of Physiology - Heart and Circulatory Physiology. - 2004. - Vol. 287. - P. 645-651.Berdyyeva, T. K. Human epithelial cells increase their rigidity with ageing in vitro: direct measurements / T. K. Berdyyeva, C. D. Woodworth, I. Sokolov // Physics in Medicine and Biology. - 2005. - Vol. 7, № 50(1). - P. 81-92.Berdyyeva, T. K. Human epithelial cells increase their rigidity with ageing in vitro: direct measurements / T. K. Berdyyeva, C. D. Woodworth, I. Sokolov // Physics in Medicine and Biology. - 2005. - Vol. 7, № 50(1). - P. 81-92.Stiffness of normal and pathological erythrocytes studied by means of atomic force microscopy / I. Dulinska [et al.] // Journal of Biochemical and Biophysical Methods. - 2006. - Vol. 66. - P. 1-11.Stiffness of normal and pathological erythrocytes studied by means of atomic force microscopy / I. Dulinska [et al.] // Journal of Biochemical and Biophysical Methods. - 2006. - Vol. 66. - P. 1-11.Стародубцева, М. Н. Пероксинитрит-индуцированные структурные перестройки в эритроцитарных мембранах / М. Н. Стародубцева, Т. Г. Кузнецова, Т. А. Кузнецова // Весцi НАН Беларусi, серия биологических наук. - 2006. - № 3. - С. 96-99.Стародубцева, М. Н. Пероксинитрит-индуцированные структурные перестройки в эритроцитарных мембранах / М. Н. Стародубцева, Т. Г. Кузнецова, Т. А. Кузнецова // Весцi НАН Беларусi, серия биологических наук. - 2006. - № 3. - С. 96-99.Стародубцева, М. Н. Механические свойства эритроцитарных мембран при действии пероксинитрита / М. Н. Стародубцева, Т. Г. Кузнецова, С. Н. Черенкевич // Бюллетень экспериментальной биологии и медицины. - 2007. - Т. 143, № 2. - С. 222-230.Стародубцева, М. Н. Механические свойства эритроцитарных мембран при действии пероксинитрита / М. Н. Стародубцева, Т. Г. Кузнецова, С. Н. Черенкевич // Бюллетень экспериментальной биологии и медицины. - 2007. - Т. 143, № 2. - С. 222-230.Структурно-механические свойства мембран эритроцитов больных сахарным диабетом второго типа. / М. Н. Стародубцева [и др.] // Бюллетень экспериментальной биологии и медицины. - 2008.Структурно-механические свойства мембран эритроцитов больных сахарным диабетом второго типа. / М. Н. Стародубцева [и др.] // Бюллетень экспериментальной биологии и медицины. - 2008.Rosenbluth, M. J. Force Microscopy of Nonadherent Cells: A Comparison of Leukemia Cell Deformability / M. J. Rosenbluth, W. A. Lam, D. A. Fletcher // Biophysical Journal. - 2006. - Vol. 90. - P. 2994-3003.Rosenbluth, M. J. Force Microscopy of Nonadherent Cells: A Comparison of Leukemia Cell Deformability / M. J. Rosenbluth, W. A. Lam, D. A. Fletcher // Biophysical Journal. - 2006. - Vol. 90. - P. 2994-3003.Lekka, M. Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy / M. Lekka [et al.] // European Biophysics Journal. - 1999. - Vol. 28(4). - P. 312-316.Lekka, M. Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy / M. Lekka [et al.] // European Biophysics Journal. - 1999. - Vol. 28(4). - P. 312-316.Lekka, M. The effect of chitosan on stiffness and glycolytic activity of human bladder cells / M Lekka. [et al] // Biochimica et Biophysica Acta 22. - 2001. - Vol. 1540(2). - P. 127-136.Lekka, M. The effect of chitosan on stiffness and glycolytic activity of human bladder cells / M Lekka. [et al] // Biochimica et Biophysica Acta 22. - 2001. - Vol. 1540(2). - P. 127-136.Walch, V. Effect of streptolysin O on the microelasticity of human platelets analyzed by atomic force microscopy/ V. Walch, U. Ziegler, P. Grocurth. // Ultramicroscopy. - 2000. -Vol. 82(1-4). - P. 259-267.Walch, V. Effect of streptolysin O on the microelasticity of human platelets analyzed by atomic force microscopy/ V. Walch, U. Ziegler, P. Grocurth. // Ultramicroscopy. - 2000. -Vol. 82(1-4). - P. 259-267.Influence of Hydrocortisone on the Mechanical Properties of the Cerebral Endothelium in Vitro / S. Schrot [et al.] // Biophysical Journal. - 2005. - Vol. 89. - P. 3904-3910.Influence of Hydrocortisone on the Mechanical Properties of the Cerebral Endothelium in Vitro / S. Schrot [et al.] // Biophysical Journal. - 2005. - Vol. 89. - P. 3904-3910.

The authors declare that there are no conflicts of interest present.