zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2024-21-3-17zdor-2759Research ArticleНОВЫЕ ТЕХНОЛОГИИNEW TECHNOLOGIESРеспираторная микробиота у пациентов с недифференцированными патологическими изменениями в легкихThe respiratory microbiota in patients with undifferentiated pathological changes in the lungshttps://orcid.org/0000-0003-2133-4360МайсеенкоВ. И.MaiseenkaV. I.

Майсеенко Виктория Игоревна, старший преподаватель кафедры фтизиопульмонологии с курсом ФПКиП,

Гомель

Viktoryia I. Maiseenka, Senior Lecturer at the Department of Phthisiopulmonology with the course of Advanced Training andRetraining, 

Gomel

vikamaiseenka@gmail.comБуйневичИ. В.BuinevichI. V.

Буйневич Ирина Викторовна, к.м.н., доцент, заведующий кафедрой фтизиопульмонологии с курсом ФПКиП,

Гомель

Iryna V. Buinevich, Candidate of Medical Sciences, Associate Professor, Head of the Department of Phthisiopulmonology with the course of Advanced Training and Retraining,

Gomel

ВоропаевЕ. В.VoropaevE. V.

Воропаев Евгений Викторович, к.м.н., доцент, проректор по научной работе,

Гомель

Evgenii V. Voropaev, Candidate of Medical Sciences, Associate Professor, Vice-Rector for Scientific Work,

Gomel

ОсипкинаО. В.OsipkinaO. V.

Осипкина Ольга Викторовна, заведующий научно-исследовательской лабораторией,

Гомель

Olga V. Osipkina, Head of the Research Laboratory,

Gomel

КовалевА. А.KovalevA. A.

Ковалев Алексей Алексеевич, старший преподаватель кафедры медицинской и биологической физики,

Гомель

Alexey A. Kovalev, Senior Lecturer at the Department of Medical and Biological Physics,

Gomel

ШафоростА. С.ShaforostA. S.

Шафорост Александр Сергеевич, старший научный сотрудник научно-исследовательской лаборатории,

Гомель

Alexander S. Shaforost, Alexander S. Shaforost, Senior Researcher at the Research Laboratory,

Gomel

ЗятьковА. А.ZyatskovA. A.

Зятьков Алексей Александрович, старший научный сотрудник научно-исследовательской лаборатории,

Гомель

Alexey A. Zyatskov, Alexey A. Zyatskov, Senior Researcher at the Research Laboratory,

Gomel

УО "Гомельский государственный медицинский университет"Gomel State Medical UniversityУО «Гомельский государственный медицинский университет»Gomel State Medical University202426072024213120129Copyright © Майсеенко В.И., Буйневич И.В., Воропаев Е.В., Осипкина О.В., Ковалев А.А., Шафорост А.С., Зятьков А.А., 20242024Майсеенко В.И., Буйневич И.В., Воропаев Е.В., Осипкина О.В., Ковалев А.А., Шафорост А.С., Зятьков А.А.Maiseenka V.I., Buinevich I.V., Voropaev E.V., Osipkina O.V., Kovalev A.A., Shaforost A.S., Zyatskov A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/2759Цель исследования

Цель исследования. Изучить микробное разнообразие респираторной микробиоты у пациентов с недифференцированными патологическими изменениями в легких.

Материалы и методы

Материалы и методы. Проведено исследование биопсийного материала, полученного при проведении видеоассистированной торакоскопической резекции легких, 36 пациентов с недифференцированными патологическими изменениями в легких. Изучение микробного разнообразия легких проводили методом высокопроизводительного секвенирования, с помощью секвенатора MiSeq (Illumina, США) с использованием протокола, основанного на анализе вариабельных регионов гена 16s рРНК.

Результаты

Результаты. Изучена плотность и микробное разнообразие легочной микробиоты полученных образцов. Во всех образцах пациентов доминирующими таксонами на уровне типа являются Proteobacteria (медиана относительной представленности таксона более 90%); типы Firmicutes, Bacteroidetes, Actinobacteria представлены в меньшем количестве, а типы Fusobacteria и Cyanobacteria – только в некоторых группах. Fusobacteria встречались только у пациентов с саркоидозом легких, также только в этой группе медиана относительной представленности типа Actinobacteria была намного выше, чем в остальных (p<0,05). Во всех образцах наиболее широко представлены роды Stenotrophomonas и Delftia. Бактерия рода Pseudomonas встречалась у пациентов с раком легких

Заключение

Заключение. В результате анализа полученных данных показано, что Proteobacteria являлись значительно преобладающим типом микробиоты у пациентов с недифференцированными патологическими изменениями в легких; тип Fusobacteria встречался только у пациентов с саркоидозом легких, также только в этой группе медиана относительной представленности типа Actinobacteria была намного выше, чем в остальных группах. Наибольшее таксономическое разнообразие на уровне рода наблюдалось у пациентов с саркоидозом легких.

Objective

Objective. To study the microbial diversity of the respiratory microbiota in patients with undifferentiated pathological changes in the lungs.

Materials and methods

Materials and methods. The study of biopsy material obtained during video-assisted thoracoscopic lung resection was conducted in 36 patients with undifferentiated pathological changes in the lungs. The study of the microbial diversity of the lungs was carried out by high-performance sequencing using the MiSeq sequencer (Illumina, USA) using a protocol based on the analysis of variable regions of the 16s rRNA gene.

Results

Results. The density and microbial diversity of the pulmonary microbiota of the obtained samples were studied. In all patient samples, the dominant taxa at the Phylum level are Proteobacteria (the median relative representation of the taxon is more than 90%); the Phylums Firmicutes, Bacteroidetes, Actinobacteria are represented in smaller numbers, and the Phylums Fusobacteria and Cyanobacteria – only in some groups. Fusobacteria were found only in patients with lung sarcoidosis, and only in this group the median relative representation of the Phylum Actinobacteria was much higher than in the rest (p<0.05). The Genuses Stenotrophomonas and Delftia are the most widely represented in all samples. A bacterium of the Genus Pseudomonas has been found in patients with lung cancer. Conclusion. Proteobacteria were a significantly predominant Phylum of microbiota in patients with undifferentiated pathological changes in the lungs; Fusobacteria were found only in patients with lung sarcoidosis, and only in this group the median relative representation of the Actinobacteria were much higher than in the other groups. The greatest taxonomic diversity at the Genus level was observed in patients with lung sarcoidosis. Keywords: microbiota, lungs, tuberculosis, sarcoidosis, lung cancer> ˂ 0.05). The Genuses Stenotrophomonas and Delftia are the most widely represented in all samples. A bacterium of the Genus Pseudomonas has been found in patients with lung cancer.

Conclusion

Conclusion. Proteobacteria were a significantly predominant Phylum of microbiota in patients with undifferentiated pathological changes in the lungs; Fusobacteria were found only in patients with lung sarcoidosis, and only in this group the median relative representation of the Actinobacteria were much higher than in the other groups. The greatest taxonomic diversity at the Genus level was observed in patients with lung sarcoidosis.

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The authors declare that there are no conflicts of interest present.