zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2024-21-3-05zdor-2730Research ArticleКЛИНИЧЕСКАЯ МЕДИЦИНАCLINICAL MEDICINEИзменения микробиома кишечника у детей, страдающих атопическим дерматитомChanges in the gut microbiome in children with atopic dermatitisПорошинаЛ. А.ParoshynaL. A.

Порошина Лариса Александровна, старший преподаватель кафедры внутренних болезней № 2 с курсом ФПКиП,

Гомель

Larysa A. Paroshyna, Senior Lecturer at the Department of Internal Medicine №2,

Gomel

plary@mail.ruСадченкоПолина СергеевнаS.Sadchenko P.

Садченко Полина Сергеевна, студентка 4 курса лечебного факультета, 

Гомель

Polina S. Sadchenko, Student, 

Gomel

Sadchenko.polina@gmail.comУО "Гомельский государственный медицинский университет"Gomel State Medical University2024220720242134044Copyright © Порошина Л.А., Садченко П.С., 20242024Порошина Л.А., Садченко П.С.Paroshyna L.A., S. S.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/2730Цель исследования

Цель исследования. Определить особенности видового состава и количественных соотношений микроорганизмов в кишечнике у пациентов с атопическим дерматитом.

Материалы и методы

Материалы и методы. У 50 детей с атопическим дерматитом в возрасте от 1 до 17 лет проведено микробиологическое исследования кала для определения видового состава и количественных соотношений микроорганизмов в кишечнике.

Результаты

Результаты. У 84 % детей, страдающих атопическим дерматитом, выявлено изменение видового состава и количественных соотношений микроорганизмов в кишечнике. Снижение количества лактобактерий и/или бифидобактерий наблюдалось у половины пациентов, изменение качественного и количественного состава кишечной палочки определялось у 50 % детей с атопическим дерматитом: I степень тяжести дисбиоза кишечника была выявлена у 5 (10 %) пациентов с атопическим дерматитом, II степень — у 16 (32 %) человек, III степень — у 21 (42 %) ребенка.

Заключение

Заключение. У детей, страдающих атопическим дерматитом, выявлена частая встречаемость дисбиоза кишечника преимущественно за счет снижения основных представителей облигатной микрофлоры кишечника, бифидо- и лактобактерий, а также за счет изменения качественного и количественного состава кишечной палочки.

Objective

Objective. To determine the peculiarities of species composition and quantitative ratios of microorganisms in the intestine of patients with atopic dermatitis.

Materials and methods

Materials and methods. Fecal microbiologic examination was carried out in 50 children with atopic dermatitis aged from 1 to 17 years to determine the species composition and quantitative ratios of microorganisms in the intestine.

Results

Results. The change in the species composition and quantitative ratios of microorganisms in the intestine was detected in 84% of children with atopic dermatitis. Decreases in the number of lactobacilli and/or bifidobacteria were observed in half of the patients, changes in the qualitative and quantitative composition of Escherichia coli were detected in 50% of children with atopic dermatitis. The I severity level of intestinal dysbiosis was detected in 5 (10%) patients with atopic dermatitis, II level - in 16 (32%) patients, III level - in 21 (42%) children.

Conclusion

Conclusion. In children suffering from atopic dermatitis the frequent occurrence of intestinal dysbiosis was revealed mainly due to the decrease of the main representatives of the obligate intestinal microflora, bifido- and lactobacilli, as well as due to changes in the qualitative and quantitative composition of Escherichia coli. 

лактобактериибифидобактериикишечная палочказолотистый стафилококкlactobacillibifidobacteriaEscherichia coliStaphylococcus aureusReferencesOlejniczak-Staruch I, Ciążyńska M, Sobolewska-Sztychny D, Narbutt J, Skibińska M, Lesiak A. Alterations of the Skin and Gut Microbiome in Psoriasis and Psoriatic Arthritis. Int J Mol Sci. 2021;22(8):3998. DOI: https://doi.org/10.3390/ijms22083998Olejniczak-Staruch I, Ciążyńska M, Sobolewska-Sztychny D, Narbutt J, Skibińska M, Lesiak A. Alterations of the Skin and Gut Microbiome in Psoriasis and Psoriatic Arthritis. Int J Mol Sci. 2021;22(8):3998. DOI: https://doi.org/10.3390/ijms22083998Myers B, Brownstone N, Reddy V, Chan S, Thibodeaux Q, Truong A, et al. The gut microbiome in psoriasis and psoriatic arthritis. Pract Res Clin Rheumatol. 2019;33(6):101494. DOI: https://doi.org/10.1016/j.berh.2020.101494Myers B, Brownstone N, Reddy V, Chan S, Thibodeaux Q, Truong A, et al. The gut microbiome in psoriasis and psoriatic arthritis. Pract Res Clin Rheumatol. 2019;33(6):101494. DOI: https://doi.org/10.1016/j.berh.2020.101494Стома И.О., Карпов И.А. Микробиом человека. Минск: ДокторДизайн; 2018. 122 с.Stoma IO, Karpov IA. Microbiome of the human being. Minsk: DoctorDesign; 2018. 122 p.Thye AY-K; Bah YR, LawJ.W-F, Tan LT-H, He Y-W, Wong S-H, et al. Gut–Skin Axis: Unravelling the Connection between the Gut Microbiome and Psoriasis. Biomedicines. 2022:1037. DOI: https://doi.org/10.3390/biomedicines10051037Thye AY-K; Bah YR, LawJ.W-F, Tan LT-H, He Y-W, Wong S-H, et al. Gut–Skin Axis: Unravelling the Connection between the Gut Microbiome and Psoriasis. Biomedicines. 2022:1037. DOI: https://doi.org/10.3390/biomedicines10051037Fang Z, Li L, Zhang H, Zhao J, Lu W, Chen W. Gut Microbiota, Probiotics, and Their Interactions in Prevention and Treatment of Atopic Dermatitis: A Review. Front Immunol. 2021;12:720393. DOI: https://doi.org/10.3389/fimmu.2021.720393Fang Z, Li L, Zhang H, Zhao J, Lu W, Chen W. Gut Microbiota, Probiotics, and Their Interactions in Prevention and Treatment of Atopic Dermatitis: A Review. Front Immunol. 2021;12:720393. DOI: https://doi.org/10.3389/fimmu.2021.720393O’Neill CA, Monteleone G, McLaughlin JT, Paus R. The Gut-Skin Axis in Health and Disease: A Paradigm with Therapeutic Implications. Bioessays. 2016;38(11):1167-1176. DOI: https://doi.org/10.1002/bies.201600008O’Neill CA, Monteleone G, McLaughlin JT, Paus R. The Gut-Skin Axis in Health and Disease: A Paradigm with Therapeutic Implications. Bioessays. 2016;38(11):1167-1176. DOI: https://doi.org/10.1002/bies.201600008Роживанова Т.А., Полеско И.В., Щербакова М.Ю. Современные представления о микробиоценозе кожи и кишечника у больных экземой и метаболическим синдромом. Клиническая дерматология и венерология. 2015;14(2):11-16. DOI: https://doi.org/10.17116/klinderma201514211-16Rozhivanova TA, Polesko IV, Scherbakova MY. Modern ideas about the microbiocenosis of skin and intestine in patients with eczema and metabolic syndrome. Clinical dermatology and venereology. 2015;14(2):11-16. (In Russ.). DOI: https://doi.org/10.17116/klinderma201514211-16Braun C, Patra V, Lina G, Nicolas JF, Vocanson M, Nosbaum A. The role of skin dysbiosis in atopic dermatitis. Eur J Dermatol. 2022;32(4):439-444. DOI: https://doi.org/10.1684/ejd.2022.4289Braun C, Patra V, Lina G, Nicolas JF, Vocanson M, Nosbaum A. The role of skin dysbiosis in atopic dermatitis. Eur J Dermatol. 2022;32(4):439-444. DOI: https://doi.org/10.1684/ejd.2022.4289Bradley CW, Morris DO, Rankin SC, Cain CL, Misic AM, Houser T, et al. Longitudinal Evaluation of the Skin Microbiome and Association with Microenvironment and Treatment in Canine Atopic Dermatitis. J Invest Dermatol. 2016;136(6):1182-1190. DOI: https://doi.org/10.1016/j.jid.2016.01.023Bradley CW, Morris DO, Rankin SC, Cain CL, Misic AM, Houser T, et al. Longitudinal Evaluation of the Skin Microbiome and Association with Microenvironment and Treatment in Canine Atopic Dermatitis. J Invest Dermatol. 2016;136(6):1182-1190. DOI: https://doi.org/10.1016/j.jid.2016.01.023Воробьев А.А., Борисова Е.В., Моложавая О.С. Иммуносупрессивное действие патогенных грамотрицательных бактерий. Вестник РАМН. 2001;(2):21-25.Vorobyev AA, Borisova EV, Molozhavaya OS. Immunosuppressive effect of pathogenic Gram-negative bacteria. Vestnik RAMS. 2001;(2):21-25. (In Russ.).Kobayashi Т, Nagao К. Host-microbial dialogues in atopic dermatitis. International Immunology. 2019;31(7):449-456. DOI: https://doi.org/10.1093/intimm/dxz026Kobayashi Т, Nagao К. Host-microbial dialogues in atopic dermatitis. International Immunology. 2019;31(7):449-456. DOI: https://doi.org/10.1093/intimm/dxz026Hu C, van Meel ER, Medina-Gomez C, Kraaij R, Barroso M, Kiefte-de Jong J, et al. A population-based study on associations of stool microbiota with atopic diseases in schoolage children. J Allergy Clin Immunol. 2021;148(2):612-620. DOI: https://doi.org/10.1016/j.jaci.2021.04.001Hu C, van Meel ER, Medina-Gomez C, Kraaij R, Barroso M, Kiefte-de Jong J, et al. A population-based study on associations of stool microbiota with atopic diseases in schoolage children. J Allergy Clin Immunol. 2021;148(2):612-620. DOI: https://doi.org/10.1016/j.jaci.2021.04.001Zachariassen LF, Krych L, Engkilde K, Nielsen DS, Kot W, Hansen CH, et al. Sensitivity to Oxazolone Induced Dermatitis is Transferable with Gut Microbiota in Mice. Sci Rep. 2017;7:44385. DOI: https://doi.org/10.1038/srep44385Zachariassen LF, Krych L, Engkilde K, Nielsen DS, Kot W, Hansen CH, et al. Sensitivity to Oxazolone Induced Dermatitis is Transferable with Gut Microbiota in Mice. Sci Rep. 2017;7:44385. DOI: https://doi.org/10.1038/srep44385Kalliomaki M, Kirjavainen P, Eerola E. Distinct patterns of neonatal gut microflora in infants developing or not developing atopy. J Allergy Clinical Immunology. 2016;107:129-134. DOI: https://doi.org/10.1067/mai.2001.111237Kalliomaki M, Kirjavainen P, Eerola E. Distinct patterns of neonatal gut microflora in infants developing or not developing atopy. J Allergy Clinical Immunology. 2016;107:129-134. DOI: https://doi.org/10.1067/mai.2001.111237Ouwehand AC, Isolauri E, He F, Hashimoto H, Benno Y, Salminen S. Differences in Bifidobacterium flora composition in allergic and healthy infants. Journal of Allergy and Clinical Immunology. 2001;108:144-145. DOI: https://doi.org/10.1067/mai.2001.115754Ouwehand AC, Isolauri E, He F, Hashimoto H, Benno Y, Salminen S. Differences in Bifidobacterium flora composition in allergic and healthy infants. Journal of Allergy and Clinical Immunology. 2001;108:144-145. DOI: https://doi.org/10.1067/mai.2001.115754Burcelin R, Serino M, Chabo C, Blasco-BaqueV, Amar J. Gut microbiota and diabetes: from pathogenesis to therapeutic perspective. Acta Diabetol. 2011;48(4):257-273. DOI: https://doi.org/10.1007/s00592-011-0333-6Burcelin R, Serino M, Chabo C, Blasco-BaqueV, Amar J. Gut microbiota and diabetes: from pathogenesis to therapeutic perspective. Acta Diabetol. 2011;48(4):257-273. DOI: https://doi.org/10.1007/s00592-011-0333-6Солдаткин П.К. Дисбактериоз кишечника: учебное пособие. Благовещенск; 2015. 44 с.Soldatkin PK. Intestinal dysbacteriosis: textbook. Blagoveshchensk; 2015. 44 р. (In Russ.).

The authors declare that there are no conflicts of interest present.