zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2021-18-3-21zdor-2047Research ArticleНОВЫЕ ТЕХНОЛОГИИNEW TECHNOLOGIESЗначение определения GPER1, HO-1, HO-2 в эндометрии у пациенток с хроническим эндометритомImportance of GPER1, HO-1, HO-2 expression in the endometrium in patients with chronic endometritishttps://orcid.org/0000-0002-8465-9368ЛызиковаЮ. А.LyzikovaYu. A.

Лызикова Юлия Анатольевна, к.м.н., доцент, доцент кафедры акушерства и гинекологии с курсом ФПКиП 

Гомель

Yuliya A. Lyzikova, PhD (Med), Associate Professor, Associate Professor at the Department of Obstetrics and Gynecology with the course of the Faculty of Professional Development and Retraining 

Gomel

lyzikovayulia@yandex.byhttps://orcid.org/0000-0002-3808-8832ЗиновкинД. А.ZinovkinD. A.

Зиновкин Дмитрий Александрович, ассистент кафедры патологической анатомии 

Гомель

Dmitry А. Zinovkin, Assistant Lecturer at the Department of Pathologic Anatomy

Gomel

https://orcid.org/0000-0002-9435-6109ВоропаевE. В.VoropaevE. V.

Воропаев Евгений Викторович, к.м.н., доцент, проректор по научной работе 

Гомель

Evgenii V. Voropaev, PhD (Med), Associate Professor, Vice-rector in charge of scientifc work 

Gomel

voropaev.evgenii@gmail.comГомельский государственный медицинский университетGomel State Medical University202101102021183168174Copyright © Лызикова Ю.А., Зиновкин Д.А., Воропаев E.В., 20212021Лызикова Ю.А., Зиновкин Д.А., Воропаев E.В.Lyzikova Y.A., Zinovkin D.A., Voropaev E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/2047Цель исследования

Цель исследования. Оценить экспрессию рецептора эстрогена, связанного с G-белком (GPER1) и гемоксигеназы-1 (HO-1), гемоксигеназы-2 (HO-2) у пациенток с хроническим эндометритом и определить значение указанных маркеров в диагностике заболевания.

Материалы и методы

Материалы и методы. Проанализированы результаты экспрессии GPER1 и HO-1, HO-2 у пациенток с установленным диагнозом «хронический эндометрит» и у пациенток группы сравнения.

Результаты

Результаты. Значимым для развития хронического эндометрита является экспрессия GPER1 ≤ 79 % (чувствительность — 100,00 %; специфичность — 95,83 %; +PV — 94,00 %; -PV — 100 %), экспрессия HO-1 позитивных клеток > 5 клеток (чувствительность — 100,00 %; специфичность —100%; +PV — 100 %; -PV — 100 %). Железы эндометрия у пациенток с хроническим эндометритом экспрессируют маркеры воспаления HO-1 и HO-2 значимо более часто, чем в группе сравнения (p < 0,001).

Заключение

Заключение. Представленные данные указывают на нарушения ферментов метаболизма железа и неканонических путей рецепции эстрогена при хроническом эндометрите. Определение экспрессии GPER1, HO-1, HO-2 в эндометрии у пациенток с хроническим эндометритом является достоверным критерием и может быть использовано в диагностике заболевания.

Objective

Objective. To evaluate the expression of G protein-coupled estrogen receptor (GPER1), heme oxygenase-1 (HO-1), and heme oxygenase-2 (HO-2) in patients with chronic endometritis and to determine the importance of these markers in the diagnosis of the disease.

Materials and methods

Materials and methods. The results of GPER1, HO-1, and HO-2 expression were analyzed in patients with a confrmed diagnosis of chronic endometritis and in patients of the comparison group.

Results

Results. The expression of GPER1 ≤ 79 % (sensitivity — 100.00 %; specifcity — 95.83 %; +PV — 94.00 %; -PV — 100 %), the expression of HO-1 positive cells > 5 % (sensitivity — 100.00 %; specifcity -100%; +PV — 100 %; -PV — 100 %) are signifcant for the development of chronic endometritis. The endometrial glands in the patients with chronic endometritis express the markers of inflammation HO-1 and HO-2 signifcantly more often than in the comparison group (p < 0.001).

Conclusion

Conclusion. The presented data indicate defects of the enzymes of iron metabolism and non-canonical pathways of estrogen reception in chronic endometritis. The determination of GPER1, HO-1, HO-2 expression in the endometrium in patients with chronic endometritis is a reliable criterion and may be used in the diagnosis of the disease.

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[дата обращения 2021 июнь 05]. Режим доступа: https://journal.gsmu.by/jour/article/view/93Lyzikova YuA, Zinovkin DA. The clinical description of infertile female patients and immunohistochemical evaluation of FoxP3 andCD56 expression in the endometrium. [Electronic resource]. Health and Ecology Issues. 2019;(1):35-40. [date of access 2021 June 05]. Available from: https://journal.gsmu.by/jour/article/view/93 (in Russ.).

The authors declare that there are no conflicts of interest present.