zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2021-18-2-21zdor-1954Research ArticleНОВЫЕ ТЕХНОЛОГИИNEW TECHNOLOGIESМолекулярно-генетические маркеры риска развития хронизации головной боли напряженного типа и мигрениMolecular genetic markers of the risk of tension-type headache and migraine chronization developmenthttps://orcid.org/0000-0002-3252-2626КостюкС. А.KostiukS. A.

Костюк Светлана Андреевна, д.м.н., профессор, главный научный сотрудник Научно-исследовательской лаборатории

Минск

Svetlana A. Kostiuk, DMedSc, Professor, Chief Researcher at the Science-Research Laboratory

Minsk

s.kostiuk@mail.ruhttps://orcid.org/0000-0001-7130-2776ПолуянО. С.PoluyanO. S.

Полуян Ольга Сергеевна, к.б.н., ведущий научный сотрудник Научно-исследовательской лаборатории

Минск

Olga S. Poluyan, PhD (Biol), Leading Researcher at the Science-Research Laboratory

Minsk

olga.poluyan@mail.ruhttps://orcid.org/0000-0003-1044-5401СимирскийМ. В.SimirskiM. V.

Симирский Максим Викторович, студент

Минск

Maxim V. Simirski, student

Minsk

simirski@mail.ruhttps://orcid.org/0000-0001-6851-1016МарьенкоИ. П.MarjenkoI. P.

Марьенко Ирина Павловна, к.м.н., ведущий научный сотрудник неврологического отдела

Минск

Irina P. Marjenko, PhD (Med), Leading Researcher at the Science-Research Laboratory of the Neurological Department

Minsk

iramaryenko@gmail.comБелорусская медицинская академия последипломного образованияBelarusian Medical Academy of Post-Graduate EducationБелорусский государственный медицинский университетBelarusian State Medical UniversityРеспубликанский научно-практический центр неврологии и нейрохирургииRepublican Research and Clinical Center of Neurology and Neurosurgery202130062021182147154Copyright © Костюк С.А., Полуян О.С., Симирский М.В., Марьенко И.П., 20212021Костюк С.А., Полуян О.С., Симирский М.В., Марьенко И.П.Kostiuk S.A., Poluyan O.S., Simirski M.V., Marjenko I.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/1954Цель

Цель: установить молекулярно-генетические критерии риска хронизации головной боли напряженного типа и мигрени.

Материалы и методы

Материалы и методы. Детекцию результатов по определению аллельных вариантов проводили методом горизонтального электрофореза с использованием маркера молекулярных масс. Определение генотипов полиморфных вариантов генов проводили с применением метода анализа кривых плавления продуктов ПЦР высокого разрешения.

Результаты

Результаты. На основании проведенных молекулярно-генетических исследований установлено, что статистически значимыми (p < 0,05) достоверными факторами риска хронизации головной боли напряженного типа являются: выявление А-аллеля и AA-генотипа полиморфизма DBH3 гена дофамин-бета-гидроксилазы DBH, а также выявление G-аллеля и GG-генотипа полиморфизма Intron3SNP гена препротахикинина TAC1. Установлено, что статистически значимыми (p < 0,05) достоверными факторами риска хронизации мигрени являются: выявление А-аллеля, GA- и АА-генотипов полиморфизма G29A гена транспортера серотонина SLC6A4, а также выявление G-аллеля и GG-генотипа полиморфизма rs7793277 гена препротахикинина TAC1.

Заключение

Заключение. Выявление в сыворотке крови указанных полиморфизмов генов дофамина и препротахикинина увеличивает риск хронизации головной боли напряженного типа в 1,395–1,991 раза, риск хронизации мигрени — в 1,235–1,395 раза.

Objective

Objective: to identify the molecular genetic criteria of the risk of tension-type headache and migraine chronization development.

Materials and methods

Materials and methods. The detection of the results for the determination of allelic variants was carried out by means of horizontal electrophoresis using a molecular weight marker. The determination of the genotypes of the polymorphic variants of genes was carried out using high resolution melting PCR analysis.

Results

Results. Based on the performed molecular genetic studies, it has been established that the statistically significant (p < 0.05) risk factors of tension-type headache chronization are: the identification of the A-allele and AA-genotype of the DBH3 polymorphism of the dopamine-beta-hydroxylase gene DBH, as well as the identification of the G-allele and the GG-genotype of the Intron3SNP polymorphism of the preprotachykinin gene TAC1. It has been found that the statistically significant (p < 0.05) risk factors of migraine chronization are: the identification of the A-allele, GA- and AA-genotypes of the G29A polymorphism of the serotonin transporter gene SLC6A4, as well as the identification of the G-allele and the GG-genotype of the rs7793277 polymorphism of the preprotachykinin gene TAC1.

Conclusion

Conclusion. The detection of these polymorphisms of the dopamine and preprotachykinin genes in the blood serum increases the risk of tension headache chronization by 1.395–1.991 times; the risk of migraine chronization by 1.235–1.395 times.

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The authors declare that there are no conflicts of interest present.