zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2015-12-4-5zdor-1619Research ArticleКЛИНИЧЕСКАЯ МЕДИЦИНАCLINICAL MEDICINEРОЛЬ ПОЛИМОРФИЗМА Т251А ГЕНА IL-8 В ПАТОГЕНЕЗЕ ЗАБОЛЕВАНИЙ ЖЕЛУДКАTHE ROLE OF IL-8 (Т251А) GENE POLYMORPHISM IN THE PATHOGENESIS OF GASTRIC DISEASESВоропаеваА. В.VoropayevaA. V.noemail@neicon.ruРеспубликанский научно-практический центр радиационной медицины и экологии человекаRepublican Research Centre for Radiation Medicine and Human Ecology201528122015042932Copyright © Воропаева А.В., 20152015Воропаева А.В.Voropayeva A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/1619

Цель: оценить значимость полиморфизма гена IL-8 (Т251А) в патогенезе заболеваний желудка. Материалы и методы. В исследование включено 250 пациентов с диагнозом «Хронический гастрит» и 72 пациента с диагнозом «Рак желудка». Для определения полиморфизма гена IL-8 (Т251А), выявления ДНК H. pylori и СаgA генотипа использовали метод аллельспецифичной полимеразной цепной реакции (АС-ПЦР). Результаты. Наличие у пациента генотипа ТА IL-8 (T251A) повышает риск развития рака желудка в 2 раза независимо от статуса инфицированности СagA положительными штаммами H. pylori (р = 0,006). Заключение. Генотип ТА IL-8 (T251A) значимо чаще встречался при РЖ: χ2 = 0,006; ОШ - 2,07; 95 % ДИ 1,22-3,53. Диагностическая чувствительность определения данного генотипа составляет 55,6 %, диагностическая специфичность - 62,4. Наличие достоверных различий в распределении аллелей и генотипов свидетельствует об ассоциации полиморфного маркера с заболеванием, в данном случае наличие у пациента генотипа ТА IL-8 (T251A) повышает риск развития рака желудка в 2 раза. При изучении взаимосвязи между полиморфизмом IL-8 (T251A) и атрофией желудка достоверных различий не выявлено (χ2 = 0,249). Статистически недостоверны и различия между степенью активности гастрита и полиморфизмом IL-8 (T251A) (χ2 = 0,341). Также не установлена взаимосвязь между полиморфизмом IL-8 (T251A) и инфицированностью СagA+ штаммами H. pylori в обеих изучаемых группах - χ2 = 0,188 в группе ХГ и χ2 = 0,181 в группе РЖ.

Objective: to assess the role of IL-8 (Т251А) gene polymorphism in the pathogenesis of gastric diseases. Materials and methods. The study included 250 patients diagnosed with chronic gastritis and 72 patients diagnosed with gastric cancer. To define IL-8 (Т251А) gene polymorphism, and detect the DNA of H. pylori and СаgA genotype, we used the method of allel-specific polymerase chain reaction (AS PCR). Results. The presence of ТА IL-8 genotype (T251A) in a patient increases twice as much the risk for gastric cancer regardless of the status of СagA infection with H. pylori positive strains (р = 0.006). Conclusion. ТА IL-8 (T251A) genotype was significantly more prevalent in gastric cancer: χ2 = 0.006; OR - 2.07; 95 % CI 1.22-3.53. The diagnostic sensitivity of detection of this genotype makes up 55.6 %, the diagnostic specificity - 62.4. The presence of reliable differences in the distribution of alleles and genotypes is indicative of the association of the polymorphic marker with the illness, in this particular case, the presence of ТА IL-8 (T251A) genotype in a patient increases twice as much the risk for the development of gastric cancer. While studying the interrelations between IL-8 (T251A) polymorphism and stomach atrophy we revealed no reliable differences (χ2 = 0.249). The differences between the activity of gastritis and IL-8 (T251A) (χ2 = 0.341) polymorphism are statistically unreliable. No interrelation between IL-8 (T251A) polymorphism and СagA+ infection with H. pylori strains in both the studied groups was found: χ2 =0.188 in the group with chronic gastritis and χ2 =0.181 in the group with gastric cancer.

H. pyloriгенотипаллелиинтерлейкин-8рак желудкагастритH. pylorigenotypeallelesinterleukin-8gastric cancergastritisReferencesPathogenesis of Helicobacter pylori infection / J. G. Kusters [et al.] // Clin Microbiol Rev. - 2006. - Vol. 19, № 3. - P. 449-490.Pathogenesis of Helicobacter pylori infection / J. G. Kusters [et al.] // Clin Microbiol Rev. - 2006. - Vol. 19, № 3. - P. 449-490.Cytokines, cytokine gene polymorphisms and Helicobacter pylori infection: Friend or foe? / C. Figueiredo [et al.] // World J Gastroenterol. - 2014. - Vol. 20, Is. 18. - P. 5235-5243.Cytokines, cytokine gene polymorphisms and Helicobacter pylori infection: Friend or foe? / C. Figueiredo [et al.] // World J Gastroenterol. - 2014. - Vol. 20, Is. 18. - P. 5235-5243.Genotyping of IL-8-251T>A yields prognostic information in patients with gastric carcinoma / C. Xiuyu [et al.] // Biomarkers. - 2013. - Vol. 18, Is. 7. - P. 559-564.Genotyping of IL-8-251T>A yields prognostic information in patients with gastric carcinoma / C. Xiuyu [et al.] // Biomarkers. - 2013. - Vol. 18, Is. 7. - P. 559-564.Interleukin-1 polymorphisms associated with increased risk of gastric cancer / E. M. El-Omar [et al.] // Nature. - 2000. - Vol. 404. - P. 398-402.Interleukin-1 polymorphisms associated with increased risk of gastric cancer / E. M. El-Omar [et al.] // Nature. - 2000. - Vol. 404. - P. 398-402.Increased risk of noncardia gastric cancer associated with proinflammatory cytokine gene polymorphisms / E. M. El-Omar [et al.] // Gastroenterology. - 2003. - Vol. 124. - P. 1193-1201.Increased risk of noncardia gastric cancer associated with proinflammatory cytokine gene polymorphisms / E. M. El-Omar [et al.] // Gastroenterology. - 2003. - Vol. 124. - P. 1193-1201.Association of antral mucosal levels of interleukin 8 and reactive oxygen radicals in patients infected with Helicobacter pylori / Q. B. Zhang [et al.] // Clin Sci. - 1997. - Vol. 92. - P. 69-73.Association of antral mucosal levels of interleukin 8 and reactive oxygen radicals in patients infected with Helicobacter pylori / Q. B. Zhang [et al.] // Clin Sci. - 1997. - Vol. 92. - P. 69-73.Genetic factors involved in the development of Helicobacter pylori-related gastric cancer / N. Hamajima [et al.] // Cancer Sci. - 2006. - Vol. 97. - P. 1129-1138.Genetic factors involved in the development of Helicobacter pylori-related gastric cancer / N. Hamajima [et al.] // Cancer Sci. - 2006. - Vol. 97. - P. 1129-1138.The polymorphism interleukin-8-251 A/T influences the susceptibility of Helicobacter pylori related gastric diseases in the Japanese population / M. Ohyauchi [et al.] // Gut. - 2005. - Vol. 54, № 3. - P. 330-335.The polymorphism interleukin-8-251 A/T influences the susceptibility of Helicobacter pylori related gastric diseases in the Japanese population / M. Ohyauchi [et al.] // Gut. - 2005. - Vol. 54, № 3. - P. 330-335.The interleukin-8-251 A allele is associated with increased risk of noncardia gastric adenocarcinoma in Helicobacter pylori-infected Koreans / B. D Ye [et al.] // J. Clin. Gastroenterol. - 2009. - Vol. 43, № 3. - P. 233-239.The interleukin-8-251 A allele is associated with increased risk of noncardia gastric adenocarcinoma in Helicobacter pylori-infected Koreans / B. D Ye [et al.] // J. Clin. Gastroenterol. - 2009. - Vol. 43, № 3. - P. 233-239.Genetic factors involved in the development of Helicobacter pylori-related gastric cancer / N. Hamajima [et al.] // Cancer Sci. - 2006. - Vol. 97. - P. 1129-1138.Genetic factors involved in the development of Helicobacter pylori-related gastric cancer / N. Hamajima [et al.] // Cancer Sci. - 2006. - Vol. 97. - P. 1129-1138.Helicobacter pylori seropositivity and cytokine gene polymorphisms / Y. Saijo [et al.] // World J. Gastroenterol. - 2007. - Vol. 13. - P. 4445-4451.Helicobacter pylori seropositivity and cytokine gene polymorphisms / Y. Saijo [et al.] // World J. Gastroenterol. - 2007. - Vol. 13. - P. 4445-4451.

The authors declare that there are no conflicts of interest present.