zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2012-9-2-1zdor-1243Research ArticleОБЗОРЫ И ЛЕКЦИИREVIEWS AND LECTURESРОЛЬ СТВОЛОВЫХ КЛЕТОК В РЕГЕНЕРАЦИИ ПЕЧЕНИ И ПЕРСПЕКТИВЫ ИХ ИСПОЛЬЗОВАНИЯ В ЛЕЧЕНИИ ПЕЧЕНОЧНОЙ НЕДОСТАТОЧНОСТИ (обзор литературы)THE ROLE OF STEM CELLS IN LIVER REGENERATION AND PROSPECTS OF THEIR USE IN THE TREATMENT OF LIVER IMPAIRMENT (literature review)ЛызиковА. Н.LyzikovA. N.noemail@neicon.ruСкуратовА. Г.SkuratovA. G.noemail@neicon.ruВоропаевЕ. В.VoropayevE. V.noemail@neicon.ruПризенцовА. А.PrizentsovA. A.noemail@neicon.ruГомельский государственный медицинский университетGomel State Medical University20122806201202713Copyright © Лызиков А.Н., Скуратов А.Г., Воропаев Е.В., Призенцов А.А., 20122012Лызиков А.Н., Скуратов А.Г., Воропаев Е.В., Призенцов А.А.Lyzikov A.N., Skuratov A.G., Voropayev E.V., Prizentsov A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/1243

Цель исследования: провести аналитический обзор литературы, посвященной изучению роли стволовых клеток в регенерации печени и перспективам клеточной трансплантации при печеночной недостаточности. Материалы и методы: публикации современных зарубежных и отечественных авторов, интернет-ресурсы PubMed. Результаты: рассмотрены патогенетические основы регенерации печени и достижения терапии печеночной недостаточности с помощью стволовых клеток в экспериментах на животных с индуцированным заболеванием печени и в клинических испытаниях, а также дальнейшие перспективы их использования. Заключение: цирроз печени и печеночная недостаточность остаются одной из основных причин смерти пациентов во всем мире. Наиболее эффективным методом лечения тяжелых болезней печени является ее трансплантация. Однако дефицит донорских органов и высокий риск отторжения трансплантата являются основными проблемами трансплантации печени. Альтернативным методом трансплантации печени является заселение печени стволовыми клетками и изолированными гепатоцитами. Остаются открытыми вопросы и нерешенные проблемы клеточной трансплантации, которые требуют дальнейших исследований.

The aim of the study: to carry out the analytical literature review on the study of stem cells role in liver regeneration and prospects of cell transplantation in patients with liver impairment. Materials and methods: medical publications of modern foreign and national authors, Internet resources PubMed. Results: consideration of pathogenetic basis of liver regeneration and achievement of liver impairment management with stem cells in animals with induced liver disease and in clinical trials as well as future prospects. Conclusion: liver cirrhosis and liver impairment remain one of the main causes of death in patients around the world. The most effective treatment for severe liver disease is its transplantation. However, the deficiency of donor organs and high risk of the transplant rejection are the main problems of liver transplantation. An alternative method of liver transplantation is the repopulation of liver with stem cells and isolated hepatocytes. But there are still unresolved problems of cell transplantation, which require further research.

цирроз печенипеченочная недостаточностьстволовые клеткиклеточная транплантацияliver cirrhosisliver failurestem cellscell transplantationReferencesДолгих, М. С. Перспективы терапии печеночной недостаточности с помощью стволовых клеток / М. С. Долгих // Биомедицинская химия. - 2008. - Т. 54, Вып. 4. - С. 376-391.Долгих, М. С. Перспективы терапии печеночной недостаточности с помощью стволовых клеток / М. С. Долгих // Биомедицинская химия. - 2008. - Т. 54, Вып. 4. - С. 376-391.Кирик, В. М. Стволовые клетки из жировой ткани: основные характеристики и перспективы клинического применения в регенеративной медицине / В. М. Кирик, Г. М. Бутенко // Журн. АМН України. - 2010. - Т. 16, № 4. - С. 576-604.Кирик, В. М. Стволовые клетки из жировой ткани: основные характеристики и перспективы клинического применения в регенеративной медицине / В. М. Кирик, Г. М. Бутенко // Журн. АМН України. - 2010. - Т. 16, № 4. - С. 576-604.Яргин, С. В. Стволовые клетки и клеточная терапия: на подступах к научному подходу / С. В. Яргин // Цитология. - 2010. - Т. 52, № 11. - С. 918-920.Яргин, С. В. Стволовые клетки и клеточная терапия: на подступах к научному подходу / С. В. Яргин // Цитология. - 2010. - Т. 52, № 11. - С. 918-920.Hepatocytes from non-hepatic adult stem cells / M. R. Alison [et al.] // Nature. - 2000. - Vol. 406. - P. 257-259.Hepatocytes from non-hepatic adult stem cells / M. R. Alison [et al.] // Nature. - 2000. - Vol. 406. - P. 257-259.Isolation, characterization and transplantation of bone marrow-derived hepatocyte stem cells / I. Avital [et al.] // Bioch. Biophys. Res. Communic. - 2001. - Vol. 288. - P. 156-164.Isolation, characterization and transplantation of bone marrow-derived hepatocyte stem cells / I. Avital [et al.] // Bioch. Biophys. Res. Communic. - 2001. - Vol. 288. - P. 156-164.Characterization of human adult stem cell populations isolated from visceral and subcutaneous adipose tissue / S. Baglioni [et al.] // FASEB J. - 2009. - Т. 23, № 10. - P. 3494-3505.Characterization of human adult stem cell populations isolated from visceral and subcutaneous adipose tissue / S. Baglioni [et al.] // FASEB J. - 2009. - Т. 23, № 10. - P. 3494-3505.Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes / A. Banas [et al.] // Hepatology. - 2007. - Vol. 46. - P. 219-228.Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytes / A. Banas [et al.] // Hepatology. - 2007. - Vol. 46. - P. 219-228.Bone marrow stromal stem cells nature, biology and potential applications / P. Bianco [et al.] // Stem Cells. - 2001. - Vol. 19. - P. 180-192.Bone marrow stromal stem cells nature, biology and potential applications / P. Bianco [et al.] // Stem Cells. - 2001. - Vol. 19. - P. 180-192.Dan, Y. Y. Liver stem cells: a scientific and clinical perspective / Y. Y. Dan, G. C. Yeoh // J. Gastroenterol. Hepatol. - 2008. - № 23. - P. 687-698.Dan, Y. Y. Liver stem cells: a scientific and clinical perspective / Y. Y. Dan, G. C. Yeoh // J. Gastroenterol. Hepatol. - 2008. - № 23. - P. 687-698.Surface markers for the murine oval cell response / C. Dorrell [et al.] // Hepatology. - 2008. - № 48. - Р. 1282-1291.Surface markers for the murine oval cell response / C. Dorrell [et al.] // Hepatology. - 2008. - № 48. - Р. 1282-1291.Fausto, N. Liver regeneration and repair: hepatocytes, progenitor cells, and stem cells / N. Fausto // Hepatology. - 2004. - № 39. - Р. 1477-1487.Fausto, N. Liver regeneration and repair: hepatocytes, progenitor cells, and stem cells / N. Fausto // Hepatology. - 2004. - № 39. - Р. 1477-1487.Locating the stem cell niche and tracing hepatocyte lineages in human liver / T. G. Fellous [et al.] // Hepatology. - 2009. - № 49. - Р. 1655-1663.Locating the stem cell niche and tracing hepatocyte lineages in human liver / T. G. Fellous [et al.] // Hepatology. - 2009. - № 49. - Р. 1655-1663.Liver specific gene expression in cultured human hematopoietic stem cells / H. C. Fiegel [et al.] // Stem Cells. - 2003. - Vol. 21, № 1. - P. 98-104.Liver specific gene expression in cultured human hematopoietic stem cells / H. C. Fiegel [et al.] // Stem Cells. - 2003. - Vol. 21, № 1. - P. 98-104.Fitzpatrick, E. Human hepatocyte transplantation: state of the art / E. Fitzpatrick, R. R. Mitry, A. Dhawan // J. Intern. Med. - 2009. - Vol. 266. - P. 339-357.Fitzpatrick, E. Human hepatocyte transplantation: state of the art / E. Fitzpatrick, R. R. Mitry, A. Dhawan // J. Intern. Med. - 2009. - Vol. 266. - P. 339-357.Reversion of hepatobiliary alterations by bone marrow transplantation in a murine model of erythropoietic hrotoporphyria / A. Fontanellas [et al.] // Hepatology. - 2000. - Vol. 32. - P. 73-81.Reversion of hepatobiliary alterations by bone marrow transplantation in a murine model of erythropoietic hrotoporphyria / A. Fontanellas [et al.] // Hepatology. - 2000. - Vol. 32. - P. 73-81.Transplanted hepatocytes engraft, survive, and proliterate in the liver of rats with carbon tetrachioride induced cirrhosis. / G. Sagandeep [et al.] // J. Pathol. - 2000. - Vol. 191. - P. 78-85.Transplanted hepatocytes engraft, survive, and proliterate in the liver of rats with carbon tetrachioride induced cirrhosis. / G. Sagandeep [et al.] // J. Pathol. - 2000. - Vol. 191. - P. 78-85.Galun, E. The role of cytokines in liver failure and regeneration, potential new molecular therapies. / E. Galun, J. H. Axelrod // Biochim. Bi.ophys. Acta. - 2002. - Vol. 1592. - P. 345-358.Galun, E. The role of cytokines in liver failure and regeneration, potential new molecular therapies. / E. Galun, J. H. Axelrod // Biochim. Bi.ophys. Acta. - 2002. - Vol. 1592. - P. 345-358.Gaudio, E. New insights into liver stem cells / E. Gaudio // Dig. Liver Dis. - 2009. - Vol. 41. - P. 455-462.Gaudio, E. New insights into liver stem cells / E. Gaudio // Dig. Liver Dis. - 2009. - Vol. 41. - P. 455-462.Herzog, E. L. Plasticity of marrow derived stem cells / E. L. Herzog, L. Chai, D. S. Krause // Blood. - 2003.-. Vol. 102. - P. 3483-3493.Herzog, E. L. Plasticity of marrow derived stem cells / E. L. Herzog, L. Chai, D. S. Krause // Blood. - 2003.-. Vol. 102. - P. 3483-3493.Pluripotency of mesenchymal stem cells derived from adult marrow / Y. Jiang [et al.] // Nature. - 2002. - Vol. 418. - P. 41-49.Pluripotency of mesenchymal stem cells derived from adult marrow / Y. Jiang [et al.] // Nature. - 2002. - Vol. 418. - P. 41-49.Human umbilical stem cell cord blood as a source of transplantable hepatic progenitor cells / S. Kakinuma [et al.] // Stem cells. - 2003. - Vol. 21. - P. 217-227.Human umbilical stem cell cord blood as a source of transplantable hepatic progenitor cells / S. Kakinuma [et al.] // Stem cells. - 2003. - Vol. 21. - P. 217-227.Katoonizadeh, A. Liver regeneration in acute severe liver impairment: a clinicopathological correlation study / A. Katoonizadeh // Liver Int. - 2006. - Vol. 26. - P. 1225-1233.Katoonizadeh, A. Liver regeneration in acute severe liver impairment: a clinicopathological correlation study / A. Katoonizadeh // Liver Int. - 2006. - Vol. 26. - P. 1225-1233.Biology of hematopoietic stem cells and progenitors implications for clinical application / M. Kondo [et al.] // Ann. Rev. Immunol. - 2003. - Vol. 21. - P. 759-806.Biology of hematopoietic stem cells and progenitors implications for clinical application / M. Kondo [et al.] // Ann. Rev. Immunol. - 2003. - Vol. 21. - P. 759-806.Hepatocytes and epithelial cells of donor origin in recipients of peripheral blood stem cells / M. Korbling [et al.] // N. Engl. Med. - 2002. - Vol. 346. № 10. - P. 738-746.Hepatocytes and epithelial cells of donor origin in recipients of peripheral blood stem cells / M. Korbling [et al.] // N. Engl. Med. - 2002. - Vol. 346. № 10. - P. 738-746.Korbling, M. Adult stem cells for tissue repair - a new therapeutic concept? / M. Korbling, Z. Estrov // N. Engl. J. Med. - 2003. - Vol. 349. - P. 570-582.Korbling, M. Adult stem cells for tissue repair - a new therapeutic concept? / M. Korbling, Z. Estrov // N. Engl. J. Med. - 2003. - Vol. 349. - P. 570-582.Kuehnle, I. The therapeutic potential of stem cells from adults / I. Kuehnle, M. A. Goodell // B.M.J. - 2002. - Vol. 325. - P. 372-376.Kuehnle, I. The therapeutic potential of stem cells from adults / I. Kuehnle, M. A. Goodell // B.M.J. - 2002. - Vol. 325. - P. 372-376.Human circulating CD14 monocytes as a source of progenitors that exhibit mesenchymal cell differentiation / M. Kuwana [et al.] // J. Leukoc. Biol. - 2003. - Vol. 74. № 5. - P. 833-845.Human circulating CD14 monocytes as a source of progenitors that exhibit mesenchymal cell differentiation / M. Kuwana [et al.] // J. Leukoc. Biol. - 2003. - Vol. 74. № 5. - P. 833-845.Adult-derived liver stem cells acquire a cardiomyocyte structural and tunctional phenotype ex vivo / B. J. Muller-Borer [et al.] // Am. J. Pathol. - 2004. - Vol. 165. № 1. - P. 135-145.Adult-derived liver stem cells acquire a cardiomyocyte structural and tunctional phenotype ex vivo / B. J. Muller-Borer [et al.] // Am. J. Pathol. - 2004. - Vol. 165. № 1. - P. 135-145.Bone marrow as a potential source of hepatic oval cells / B. E. Petersen [et al.] // Science. - 1999. - Vol. 284. - P. 1168-1170.Bone marrow as a potential source of hepatic oval cells / B. E. Petersen [et al.] // Science. - 1999. - Vol. 284. - P. 1168-1170.Reyes, M. Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells / M. Reyes, C. M. Verfailtie // Ann. NY. Acad. Sci. - 2001. - Vol. 938. - P. 231-235.Reyes, M. Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells / M. Reyes, C. M. Verfailtie // Ann. NY. Acad. Sci. - 2001. - Vol. 938. - P. 231-235.Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte like cells / R. E. Schwartz [et al.] // J. Clin. Invest. 2002. - Vol. 109. - P. 1291-1302.Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte like cells / R. E. Schwartz [et al.] // J. Clin. Invest. 2002. - Vol. 109. - P. 1291-1302.An in vivo model for monitoring trans-differentiation of bone marrow cells into functional hepatocytes / S. Terai [et al.] // Biochem. - 2003. - Vol. 134. - P. 551-558.An in vivo model for monitoring trans-differentiation of bone marrow cells into functional hepatocytes / S. Terai [et al.] // Biochem. - 2003. - Vol. 134. - P. 551-558.Liver from bone marrow in humans / N. D. Theise [et al.] // Hepatology. - 2000. - Vol. 32. - P. 11-16.Liver from bone marrow in humans / N. D. Theise [et al.] // Hepatology. - 2000. - Vol. 32. - P. 11-16.The canals of Hering and hepatic stem cells in humans / N. D. Theise [et al.] // Hepatology. - 1999. - Vol. 30. - P. 1425-1433.The canals of Hering and hepatic stem cells in humans / N. D. Theise [et al.] // Hepatology. - 1999. - Vol. 30. - P. 1425-1433.Cell fusion in the principal source of bone marrow-derived hepatocytes / X. Wang [et al.] // Nature. - 2003. - Vol. 422. - P. 897-901.Cell fusion in the principal source of bone marrow-derived hepatocytes / X. Wang [et al.] // Nature. - 2003. - Vol. 422. - P. 897-901.In vitro trans-differentiation of adult hepatic stem cells into pancreatic endocrine hormone-producing cells / L. Yang [et al.] // PNAS. - 2002. - Vol. 99, № 12. - P. 8078-8083.In vitro trans-differentiation of adult hepatic stem cells into pancreatic endocrine hormone-producing cells / L. Yang [et al.] // PNAS. - 2002. - Vol. 99, № 12. - P. 8078-8083.

The authors declare that there are no conflicts of interest present.