zdorПроблемы здоровья и экологииHealth and Ecology Issues2220-09672708-6011Gomel State Medical University10.51523/2708-6011.2011-8-2s-4zdor-1180Research ArticleБез разделаОПРЕДЕЛЕНИЕ ЭКСПРЕССИИ МАРКЕРА CD107A ДЛЯ ОЦЕНКИ ПРОТИВООПУХОЛЕВОЙ АКТИВНОСТИ ЦИТОТОКСИЧЕСКИХ ЛИМФОЦИТОВTESTING OF CD107A MARKER EXPRESSION FOR THE ASSESSMENT OF ANTITUMOR ACTIVITY OF CYTOTOXIC LYMPHOCYTESВашкевичЕ. П.VashkevichE. P.noemail@neicon.ruШманТ. В.ShmanT. V.noemail@neicon.ruРеспубликанский научно-практический центр детской онкологии и гематологииRepublican Research Center for Pediatric Oncology and Hematology20112812201102S2224Copyright © Вашкевич Е.П., Шман Т.В., 20112011Вашкевич Е.П., Шман Т.В.Vashkevich E.P., Shman T.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.gsmu.by/jour/article/view/1180

Оценка противоопухолевой активности цитотоксических клеток является важным показателем их функционального статуса при проведении клеточной иммунотерапии. В последнее время для этих целей широко используется определение экспрессии маркера CD107a методом проточной цитофлуориметрии. Проведенные нами исследования по определению противоопухолевой активности мононуклеарных клеток периферической крови (МНК), в т. ч. стимулированных интерлейкином-2, показали, что инкубация МНК в присутствии опухолевой линии К-562 способствовала достоверному повышению процента естественных киллерных (ЕК) и ЕК-подобных Т-клеток, экспрессирующих CD107a. Также была выявлена прямая корреляция между процентом CD107a+ ЕК-клеток в присутствии К-562 и количеством погибших мишеней в тесте с использованием окраски флуоресцентной метки CFSE. Метод оценки экспрессии CD107a позволяет измерять цитотоксическую активность на уровне клетки в конкретной популяции эффекторных клеток и может использоваться наряду со стандартными методами определения лизиса клеток-мишеней.

The assessment of antitumor activity of cytotoxic lymphocytes is an important indicator of their functional status in cellular immunotherapy. Testing of CD107a marker expression by the method of flow cytofluorimetry has been widely lately used for these purposes. The research that we carried out to determine the antitumor activity of mononuclear lymphocytes in peripheral blood including those stimulated by interleukine-2 showed that incubation of mononuclear lymphocytes in peripheral blood in the presence of К-562 tumor line was conducive to the reliable increase in rates of natural killer and killer-like cells expressing CD107a. A straight correlation between CD107a+killer cells percentage and the number of the dead targets in the test with the use of CFSE fluorescent label was detected. The assessment method of CD107a expression makes it possible to measure the cytotoxic activity in a cell in a concrete population of effectory cells and may be used alongside with standard methods for target-cell lysis.

мононуклеарные клеткицитотоксичностьпроточная цитофлуориметрияестественные киллерыmononuclear cellscytotoxicityflow cytofluorimetrynatural killersReferencesБИБЛИОГРАФИЧЕСКИЙ СПИСОКБИБЛИОГРАФИЧЕСКИЙ СПИСОКSensitive and viable identification of antigen-specific CD8+ T-cells by a flow cytometric assay for degranulation / M. R. Betts [et al.] // J. of Immunol. Methods. - 2003. - Vol. 281. - P. 65-78.Sensitive and viable identification of antigen-specific CD8+ T-cells by a flow cytometric assay for degranulation / M. R. Betts [et al.] // J. of Immunol. Methods. - 2003. - Vol. 281. - P. 65-78.Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labeled allogeneic target cells in vitro: inhibition by isoantibody and by drugs / K. T. Brunner [et al.] // Immunology. - 1968. - Vol. 14. - P. 181-196.Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labeled allogeneic target cells in vitro: inhibition by isoantibody and by drugs / K. T. Brunner [et al.] // Immunology. - 1968. - Vol. 14. - P. 181-196.A novel flow cytometric assay for evaluating cell-mediated cytotoxicity / M. W. Burkett [et al.] // J. Immunother. - 2005. - Vol. 28, № 4. - P. 396-402.A novel flow cytometric assay for evaluating cell-mediated cytotoxicity / M. W. Burkett [et al.] // J. Immunother. - 2005. - Vol. 28, № 4. - P. 396-402.Fisher, K. The flow cytometric PKH-26 assay for the determination of T-cell mediated cytotoxic activity / K. Fisher, A. Mackensen // Methods. - 2003. - Vol. 31. - P. 135-142.Fisher, K. The flow cytometric PKH-26 assay for the determination of T-cell mediated cytotoxic activity / K. Fisher, A. Mackensen // Methods. - 2003. - Vol. 31. - P. 135-142.New CFSE-based assay to determine susceptibility to lysis by cytotoxic T-cells of leukemic precursor cells within a heterogeneous target cell population / I. Jedema [et al.] // Blood. - 2004. - Vol. 103, № 7. - P. 2677-2682.New CFSE-based assay to determine susceptibility to lysis by cytotoxic T-cells of leukemic precursor cells within a heterogeneous target cell population / I. Jedema [et al.] // Blood. - 2004. - Vol. 103, № 7. - P. 2677-2682.Cytokine-induced killer cells against autologous CLL: direct cytotoxic effects and induction of immune accessory molecules by interferon-γ / M. Kornacker [et al.] // Int. J. Cancer. - 2006. - Vol. 119. - P. 1377-1382.Cytokine-induced killer cells against autologous CLL: direct cytotoxic effects and induction of immune accessory molecules by interferon-γ / M. Kornacker [et al.] // Int. J. Cancer. - 2006. - Vol. 119. - P. 1377-1382.Morris, D. G. Studies of lymphokine-activated killer (LAK) cells. I. Evidence using novel monoclonal antibodies that most human LAK precursor cells share a common surface marker / D. G. Morris, H. F. Pross // J. Exp. Med. - 1989. - Vol. 169. - P. 717-736.Morris, D. G. Studies of lymphokine-activated killer (LAK) cells. I. Evidence using novel monoclonal antibodies that most human LAK precursor cells share a common surface marker / D. G. Morris, H. F. Pross // J. Exp. Med. - 1989. - Vol. 169. - P. 717-736.Ex-vivo expanded human NK-cells express activating receptors that mediate cytotoxicity of allogeneic and autologous cancer cell lines by direct recognition and antibody directed cellular cytotoxicity / C. J. Voskens [et al.] // J. of Exp. Clin. Cancer Res. - 2010. - Vol. 29. - P. 134-146.Ex-vivo expanded human NK-cells express activating receptors that mediate cytotoxicity of allogeneic and autologous cancer cell lines by direct recognition and antibody directed cellular cytotoxicity / C. J. Voskens [et al.] // J. of Exp. Clin. Cancer Res. - 2010. - Vol. 29. - P. 134-146.

The authors declare that there are no conflicts of interest present.